Dalli A K, Saha G, Chakraborty U
Plant Biochemistry Laboratory, Department of Botany, University of North Bengal, Siliguri 734013, India.
Indian J Exp Biol. 2007 Mar;45(3):285-90.
Methanol extract was prepared from the fronds of Pteris biaurita and partial purification was done by solvent partitioning with diethyl ether and ethyl acetate, followed by hydrolysis and further partitioning with ethyl acetate. The three fractions, thus obtained were bioassayed separately against five test fungi--Curvularia lunata, Fomes lamaoensis, Poria hypobrumea, Fuasrium oxysporum and a bacterium--Bacillus pumilus, by spore germination, radial growth and agar cup techniques. Results revealed that ethyl acetate fraction (III) contained the active principle. TLC plate bioassay of the active fraction revealed inhibition zone at an Rf of 0.5-0.65. Silica gel from this region was scraped, eluted in methanol and subjected to UV-spectrophotometric analysis. An absorption maxima of 278 nm was recorded. HPLC analysis of TLC-eluate revealed a single peak with retention time of 8.1 min. GC-MS analysis revealed six major peaks in the retention time range of 7.2-10.9 min. Comparison with GC-MS libraries revealed that the extracts may contain a mixture of eicosenes and heptadecanes.
从双角凤尾蕨的叶片中制备甲醇提取物，并通过用乙醚和乙酸乙酯进行溶剂分配进行部分纯化，随后进行水解并用乙酸乙酯进一步分配。如此获得的三个馏分分别针对五种测试真菌——新月弯孢霉、毛盖多孔菌、浅黄卧孔菌、尖孢镰刀菌和一种细菌——短小芽孢杆菌，采用孢子萌发、径向生长和琼脂杯技术进行生物测定。结果表明，乙酸乙酯馏分（III）含有活性成分。活性馏分的薄层层析板生物测定显示在Rf为0.5 - 0.65处有抑制区。刮下该区域的硅胶，用甲醇洗脱并进行紫外分光光度分析。记录到最大吸收波长为278 nm。薄层层析洗脱液的高效液相色谱分析显示有一个保留时间为8.1分钟的单峰。气相色谱 - 质谱分析显示在7.2 - 10.9分钟的保留时间范围内有六个主要峰。与气相色谱 - 质谱库比较表明，提取物可能含有二十碳烯和十七烷的混合物。
