Bie Xiao-Mei, Lü Feng-Xia, Lu Zhao-Xin, Huang Xian-Qing, Shen Juan
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Sheng Wu Gong Cheng Xue Bao. 2006 Jul;22(4):644-9.
Isolation and idenfication of lipopeptides from Bacillus subtilis fmbJ was carried out in this paper. With HPLC method, it was determined that the antimicrobial substance was composed of many components, and one of them had the similar retention time similar to surfactin. In addition, the antimicrobial substance was proved to include the closed cycle peptide bind by TLC, and one of them had the migrating rate similar to surfactin. Furthermore, ESI-MS analysis showed that the antimicrobial substance contained five homologues of fengycin, such as m/z1449.9, m/zl1463.8, m/zl1477.8, m/z1491.9 and m/z1505.9, and three homologues of surfactin, such as m/z1008.8, m/z1022.8 and m/z1036.8.
本文对枯草芽孢杆菌fmbJ的脂肽进行了分离与鉴定。采用高效液相色谱法测定该抗菌物质由多种成分组成，其中一种成分的保留时间与表面活性素相似。此外，通过薄层层析法证明该抗菌物质含有闭环肽，其中一种的迁移率与表面活性素相似。进一步的电喷雾电离质谱分析表明，该抗菌物质含有丰原素的五个同系物，如m/z1449.9、m/zl1463.8、m/zl1477.8、m/z1491.9和m/z1505.9，以及表面活性素的三个同系物，如m/z1008.8、m/z1022.8和m/z1036.8。
