Abraham G, Yadav R K, Kaushik G K
Indian J Exp Biol. 2015 Apr;53(4):232-5.
Azolla microphylla Kaulf. is an aquatic nitrogen fixing pteridophyte commonly found in aquatic habitats including paddy fields. Methanolic extract of the fronds of A. microphylla was subjected to partial purification by solvent partitioning with diethyl ether and ethyl acetate followed by hydrolysis, and further partitioning with ethyl acetate. The two fractions, thus obtained were tested for antibacterial activity. It was observed that the ethyl acetate fraction inhibited the growth of the pathogenic bacterium Xanthomonas oryzae. The GC-MS analysis of the ethyl acetate fraction showed several prominent peaks with retention time ranging from 8.83 to 45.54 min. A comparison of these peaks with the GC-MS libraries revealed that it could be eicosenes and heptadecanes with potential of antimicrobial activity.
小叶满江红（Azolla microphylla Kaulf.）是一种常见于包括稻田在内的水生栖息地的水生固氮蕨类植物。小叶满江红叶片的甲醇提取物先用乙醚和乙酸乙酯进行溶剂分配，然后水解，再用乙酸乙酯进一步分配进行部分纯化。对由此获得的两个馏分进行了抗菌活性测试。观察到乙酸乙酯馏分抑制了致病细菌水稻黄单胞菌的生长。乙酸乙酯馏分的气相色谱 - 质谱分析显示出几个保留时间在8.83至45.54分钟之间的突出峰。将这些峰与气相色谱 - 质谱库进行比较后发现，它们可能是具有抗菌活性潜力的二十碳烯和十七烷。
