Koukoulitsa Catherine, Hadjipavlou-Litina Dimitra, Geromichalos George D, Skaltsa Helen
Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Panepistimiopolis, Zografou, Athens, Greece.
J Enzyme Inhib Med Chem. 2007 Feb;22(1):99-104. doi: 10.1080/14756360600991017.
In this study, five secondary metabolites (caffeic acid, rosmarinic acid, lithospermic acid B, 12-hydroxyjasmonic acid 12-O-beta-glucoside and p-menth-3-ene-1,2-diol 1-O-beta-glucopyranoside) isolated from the polar extracts of the plant Origanum vulgare L. ssp. hirtum, were tested in vitro for their ability to inhibit soybean lipoxygenase. Among the examined compounds, lithospermic acid B demonstrated the best inhibitory activity on soybean lipoxygenase with IC50 = 0.1 mM. Docking studies have been undertaken as an attempt for better understanding the interactions of these compounds within the active site of soybean lipoxygenase. The predicted binding energy values correlated well with the observed biological data.
在本研究中，从植物牛至（Origanum vulgare L. ssp. hirtum）的极性提取物中分离出的五种次生代谢产物（咖啡酸、迷迭香酸、紫草酸B、12-羟基茉莉酸12-O-β-葡萄糖苷和对薄荷-3-烯-1,2-二醇1-O-β-吡喃葡萄糖苷）在体外测试了其抑制大豆脂氧合酶的能力。在所检测的化合物中，紫草酸B对大豆脂氧合酶表现出最佳抑制活性，IC50 = 0.1 mM。已进行对接研究，以更好地理解这些化合物在大豆脂氧合酶活性位点内的相互作用。预测的结合能值与观察到的生物学数据相关性良好。
