Molecular Chemistry, Materials and Catalysis Laboratory, Faculty of Sciences and Technologies, Sultan Moulay Slimane University, Beni-Mellal 23000, Morocco.
Team of Microbiology and Health, Laboratory of Chemistry-Biology Applied to the Environment, Faculty of Sciences, Moulay Ismail University, Zitoune, Meknes 11201, Morocco.
Molecules. 2022 Aug 15;27(16):5189. doi: 10.3390/molecules27165189.
belonging to the family Lamiaceae is widely used in food and pharmaceutical fields due to its biologically active substances. We aimed to investigate the total phenol and flavonoid contents and the phenolic composition, and to evaluate the antioxidant and antibacterial properties of hydroethanolic extract from of . Total phenol and flavonoid contents were evaluated using gallic acid and quercetin as standards, respectively, and the phenolic profile was characterized using high-performance liquid chromatography coupled to a photodiode array and electrospray ionization mass spectrometry (HPLC-PDA-ESI/MS). The antioxidant activity was determined by two methods: ferric reducing power (FRAP) assay and the phosphomolybdate method. The antibacterial effect was evaluated against four bacteria (, , and ) using the broth microdilution method. The findings show that the total phenolic and flavonoid contents were 107.789 ± 5.39 mg GAE/g dm and 14,977 ± 0.79 mg QE/g dm, respectively. A total of sixteen phenolic compounds belonging to phenolic acids and flavonoids were detected. Furthermore, the extract showed strong antioxidant activity, and displayed a bacteriostatic effect against and , and a bactericidal effect against and . Therefore, this study reveals that extracts display potential as antibacterial and natural antioxidant agents for fighting against pathogenic bacteria and preventing oxidative stress.
唇形科的由于其具有生物活性的物质而被广泛应用于食品和制药领域。本研究旨在探究水醇提取物中的总酚和总黄酮含量、酚类成分，并评估其抗氧化和抗菌性能。采用没食子酸和槲皮素分别作为标准品来评估总酚和总黄酮含量，并采用高效液相色谱-光电二极管阵列-电喷雾电离质谱联用（HPLC-PDA-ESI/MS）来对酚类成分进行分析。通过铁还原能力（FRAP）测定法和磷钼酸盐法两种方法来评估抗氧化活性。采用微量肉汤稀释法评估该提取物对四种细菌（ 、 、 、 ）的抗菌效果。研究结果表明，该提取物的总酚和总黄酮含量分别为 107.789 ± 5.39 mg GAE/g dm 和 14,977 ± 0.79 mg QE/g dm。共检测到 16 种属于酚酸和类黄酮的酚类化合物。此外，该提取物具有较强的抗氧化活性，对 和 具有抑菌作用，对 和 具有杀菌作用。因此，本研究表明 提取物具有作为天然抗氧化剂和抗菌剂的潜力，可用于对抗病原菌和预防氧化应激。
