Kawahara Yumi, Yamaoka Kaoru, Iwata Masahiro, Fujimura Masahiko, Kajiume Teruyuki, Magaki Takuro, Takeda Masaaki, Ide Toshinori, Kataoka Katsuko, Asashima Makoto, Yuge Louis
Division of Bio-Environmental Adaptation Sciences, Graduate School of Health Sciences, Hiroshima University, Hiroshima, Japan.
Pathobiology. 2006;73(6):288-94. doi: 10.1159/000099123.
In the present study, the effect of electrical stimulation was examined for the ability to induce morphological, physiological, and molecular biological effects on myoblasts during cell differentiation.
L6 rat myoblasts were electrically stimulated by newly developed methods on culture days 6, 8, 10 and 12.
This electrical stimulation accelerated the appearance of myotubes, and subsequently produced spontaneously contracting muscle fibers. Measurement of membrane potential showed that the contracting cell had functional ion channels and gap junctional intercellular communication. In the electrically stimulated cells, an enhanced expression of MyoD family and M-cadherin was also observed. Expression of connexin 43 was increased and maintained at a high level in the electrically stimulated cells.
This is the first demonstration of in vitro induction of myoblasts in spontaneously contractile muscle fibers by intermittent stimulation. This novel method for induction of myoblast differentiation represents an important advance in cell therapy.
在本研究中，检测电刺激对成肌细胞在细胞分化过程中诱导形态、生理和分子生物学效应的能力。
在培养第6、8、10和12天，采用新开发的方法对L6大鼠成肌细胞进行电刺激。
这种电刺激加速了肌管的出现，并随后产生了自发收缩的肌纤维。膜电位测量表明，收缩细胞具有功能性离子通道和间隙连接细胞间通讯。在电刺激的细胞中，还观察到MyoD家族和M-钙黏蛋白的表达增强。连接蛋白43的表达在电刺激的细胞中增加并维持在高水平。
这是首次证明通过间歇性刺激在体外诱导成肌细胞形成自发收缩肌纤维。这种诱导成肌细胞分化的新方法代表了细胞治疗的一项重要进展。
Zotero 插件