Hazarika R A, Singh D K, Kapoor K N, Agarwal R K, Pandey A B
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, India.
Indian J Exp Biol. 2007 Feb;45(2):207-11.
In the present investigation, out of 27 (24.10%) strains of Escherichia coli isolated from 112 beef samples comprising raw meat (45), kabab (36) and kofta (31), 9 (33.33%) belonging to 7 different serotypes were verotoxic as tested by vero cell cytotoxic assay. Serotype O145 was the predominant STEC in raw meat. Interestingly, one STEC-O157 strain was also detected. All the STEC strains were positive for Stx genes by polymerase chain reaction showing stx2 (77.78%) to be most predominant followed by stx1 (22.22%). Phenotypic enterohaemolysin production on washed sheep blood agar supplemented with CaCl2 revealed 6 (66.67%) STEC strains to be positive. Presence of STEC in cooked beef products, viz., kabab and kofta appeared to be a matter of concern and potential threat to public health.
在本次调查中，从112份牛肉样本（包括生肉45份、烤羊肉串36份和肉丸31份）中分离出27株大肠杆菌（占24.10%），经vero细胞细胞毒性试验检测，属于7种不同血清型的9株（占33.33%）具有产志贺毒素能力。血清型O145是生肉中主要的产志贺毒素大肠杆菌。有趣的是，还检测到一株产志贺毒素大肠杆菌O157菌株。通过聚合酶链反应检测，所有产志贺毒素大肠杆菌菌株的stx基因均呈阳性，其中stx2（占77.78%）最为常见，其次是stx1（占22.22%）。在添加氯化钙的洗涤羊血琼脂上进行表型肠溶血素检测，结果显示6株（占66.67%）产志贺毒素大肠杆菌菌株呈阳性。熟牛肉制品（即烤羊肉串和肉丸）中产志贺毒素大肠杆菌的存在似乎令人担忧，对公众健康构成潜在威胁。
