Laboratory of Plant Polysaccharides, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii Prosp., 47, 119991 Moscow, Russian Federation.
Carbohydr Res. 2010 Sep 23;345(14):2038-47. doi: 10.1016/j.carres.2010.07.009. Epub 2010 Jul 15.
The polysaccharide composition of a fucoidan preparation isolated from the brown alga Saccharina latissima (formerly Laminaria saccharina) was reinvestigated. The preparation was fractionated by anion-exchange chromatography, and the fractions obtained were analyzed by chemical methods combined with NMR spectroscopy. Several 2D procedures, including HSQC, HMQC-TOCSY, and HMQC-NOESY, were used to obtain reliable structural information from the complex spectra, and the signal assignments were additionally confirmed by comparison with the literature spectra of the related polysaccharides and synthetic oligosaccharides. In accordance with the previous data, the main polysaccharide component was shown to be a fucan sulfate containing a backbone of 3-linked alpha-l-fucopyranose residues sulfated at C-4 and/or at C-2 and branched at C-2 by single sulfated alpha-l-fucopyranose residues. In addition, three other types of sulfated polysaccharide molecules were detected in the total fucoidan preparation: (i) a fucogalactan having a backbone of 6-linked beta-d-galactopyranose residues branched mainly at C-4 and containing both terminal galactose and fucose residues; (ii) a fucoglucuronomannan having a backbone of alternating 4-linked beta-d-glucopyranosyluronic acid and 2-linked alpha-d-mannopyranose residues with alpha-l-fucopyranose residues as single branches at C-3 of alpha-d-Manp; and (iii) a fucoglucuronan having a backbone of 3-linked beta-d-glucopyranosyluronic acid residues with alpha-l-fucopyranose residues as single branches at C-4. Hence, even a single algal species may contain, at least in minor amounts, several sulfated polysaccharides differing in molecular structure. Partial resolution of these polysaccharides has been accomplished, but unambiguous evidence on their presence as separate entities was not obtained.
从褐藻 Saccharina latissima（原 Laminaria saccharina）中分离得到的褐藻糖胶制剂的多糖组成进行了重新研究。该制剂通过阴离子交换色谱进行分级，并用化学方法与 NMR 光谱相结合分析得到的级分。采用几种 2D 程序，包括 HSQC、HMQC-TOCSY 和 HMQC-NOESY，从复杂的光谱中获得可靠的结构信息，并且通过与相关多糖和合成寡糖的文献光谱进行比较，进一步确认了信号分配。根据先前的数据，主要多糖成分是一种硫酸化岩藻聚糖，其主链由 3-连接的α-L-岩藻吡喃糖残基组成，在 C-4 和/或 C-2 位硫酸化，在 C-2 位由单硫酸化的α-L-岩藻吡喃糖残基支化。此外，在总褐藻糖胶制剂中还检测到另外三种类型的硫酸化多糖分子：（i）一种岩藻半乳聚糖，其主链由 6-连接的β-D-半乳糖吡喃糖残基组成，主要在 C-4 位支化，并含有末端半乳糖和岩藻糖残基；（ii）一种岩藻甘露聚糖，其主链由交替的 4-连接的β-D-葡萄糖吡喃糖醛酸和 2-连接的α-D-甘露吡喃糖残基组成，α-L-岩藻糖残基作为α-D-Manp 的 C-3 的单支；（iii）一种岩藻葡聚糖，其主链由 3-连接的β-D-葡萄糖吡喃糖醛酸残基组成，α-L-岩藻糖残基作为 C-4 的单支。因此，即使是单一的藻类物种，也可能至少含有少量几种在分子结构上不同的硫酸化多糖。这些多糖的部分分辨率已经实现，但没有获得它们作为单独实体存在的确凿证据。
