Hemmen F, Mahana W, Jollès P, Paraf A
Laboratoire d'immunologie, INRA Tours-Nouzilly, France.
Experientia. 1992 Jan 15;48(1):79-84. doi: 10.1007/BF01923613.
Embden goose (GEWL) and Barbary duck (DEWL) egg white lysozymes possess different amino acid sequences corresponding to the g-type and c-type, respectively. GEWL was shown to be a better immunogen than DEWL in both rabbits and mice. The antigenicity of the two lysozymes was tested using different techniques (i.e. indirect ELISA, inhibition tests and immunoabsorption experiments). Injection of either GEWL or DEWL into rabbits and mice induced both specific antibodies and cross-reacting antibodies. Moreover, anti-GEWL antibodies, in contrast to anti-DEWL antibodies, did not cross-react with hen egg white lysozyme (HEWL), a c-type lysozyme. While the structure of GEWL was not modified after binding to plastic, DEWL was denaturated, but it did keep some native epitopes. It was concluded that g-type and c-type lysozymes, which have different amino acid sequences, exhibit strong common antigenic properties.
埃姆登鹅（GEWL）和巴巴里鸭（DEWL）的蛋清溶菌酶分别具有对应于g型和c型的不同氨基酸序列。在兔子和小鼠中，GEWL均被证明比DEWL是更好的免疫原。使用不同技术（即间接酶联免疫吸附测定、抑制试验和免疫吸收实验）测试了两种溶菌酶的抗原性。将GEWL或DEWL注射到兔子和小鼠体内均诱导产生特异性抗体和交叉反应抗体。此外，与抗DEWL抗体相反，抗GEWL抗体不与鸡卵清溶菌酶（HEWL，一种c型溶菌酶）发生交叉反应。虽然GEWL与塑料结合后结构未改变，但DEWL发生了变性，不过仍保留了一些天然表位。得出的结论是，具有不同氨基酸序列的g型和c型溶菌酶表现出很强的共同抗原特性。
