Cui C B, Liu H B, Gu J Y, Gu Q Q, Cai B, Zhang D Y, Zhu T J
Key Laboratory of Marine Drugs, Chinese Ministry of Education, Institute of Marine Drugs and Foods, Ocean University of China, Qingdao 266003, PR China.
Fitoterapia. 2007 Apr;78(3):238-40. doi: 10.1016/j.fitote.2006.11.017. Epub 2007 Feb 6.
Bioassay-guided fractionation of the ethyl acetate extract from the fermentation broth of marine-derived Streptomyces albogriseolus A2002 led to the isolation of echinosporin (1) and 7-deoxyechinosporin (2). Compound 1 inhibited the proliferation of tsFT210, K562 and HCT-15 cancer cells (IC(50) 91.5 microM, 25.1 microM and 247 microM respectively) and 2 showed the same effect on K562 cells (IC(50) 143 microM). Flow cytometric analysis suggested that 1 and 2 exert their anti-proliferative effects on those cells through inhibiting cell cycle at the G(2)/M phase and inducing apoptosis.
对海洋来源的白灰链霉菌A2002发酵液乙酸乙酯提取物进行生物活性导向的分离，得到了刺孢霉素（1）和7-脱氧刺孢霉素（2）。化合物1抑制tsFT210、K562和HCT-15癌细胞的增殖（IC50分别为91.5 microM、25.1 microM和247 microM），化合物2对K562细胞显示出相同的作用（IC50为143 microM）。流式细胞术分析表明，1和2通过在G2/M期抑制细胞周期和诱导凋亡对这些细胞发挥抗增殖作用。
