D'Arrigo P, Giordano C, Macchi P, Malpezzi L, Pedrocchi-Fantoni G, Servi S
Dipartimento di Chimica, Materiali ed Ingegneria Chimica Giulio Natta, Politecnico di Milano, Milano, Italy.
Int J Artif Organs. 2007 Feb;30(2):133-43. doi: 10.1177/039139880703000208.
In this work we synthesized new MDI -based poly(ether)urethanes (PEUs) with phospholipid-like residue as chain extender. Polymers were prepared by a conventional two-step solution polymerization procedure using 4,4' diphenylmethanediisocyanate (MDI) and poly(1,4- butanediol) with 1000 as molecular weight to form prepolymers which were successively polymerized with 1 glycerophosphorylcholine (1-GPC), 2-glycerophosphorylcholine (2-GPC) or glycerophosphorylserine (GPS) as chain extenders. Two reference polymers bearing 1,4-butandiol (BD) have been also synthesized. The polymers obtained were characterized by Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC) and modulated scanning calorimetry (MDSC). The biocompatibility of synthesized segmented polyurethanes was then investigated by platelet-rich plasma contact studies and related scanning electron microscopy (SEM) photographs for blood compatibility and cytotoxicity assay (MTT test) on material elution to assess the effect of any toxic leachables on cellular viability. Three polymers among all have given very satisfactory results suggesting to investigate more deeply their possible use in biomedical devices.
在本研究中，我们合成了新型的以二苯基甲烷二异氰酸酯（MDI）为基础、带有类磷脂残基作为扩链剂的聚（醚）聚氨酯（PEU）。聚合物通过传统的两步溶液聚合程序制备，使用4,4'-二苯基甲烷二异氰酸酯（MDI）和分子量为1000的聚（1,4-丁二醇）形成预聚物，然后依次与1-甘油磷酰胆碱（1-GPC）、2-甘油磷酰胆碱（2-GPC）或甘油磷酰丝氨酸（GPS）作为扩链剂进行聚合。还合成了两种带有1,4-丁二醇（BD）的参比聚合物。通过傅里叶变换红外光谱（FT-IR）、核磁共振（NMR）、差示扫描量热法（DSC）和调制扫描量热法（MDSC）对所得聚合物进行了表征。然后通过富血小板血浆接触研究以及相关的扫描电子显微镜（SEM）照片来研究合成的嵌段聚氨酯的生物相容性，以评估血液相容性和细胞毒性试验（MTT试验）对材料洗脱的影响，从而评估任何有毒可溶物对细胞活力的作用。所有聚合物中有三种给出了非常令人满意的结果，这表明需要更深入地研究它们在生物医学装置中的可能用途。
