Chao Y C, Wood H A, Chang C Y, Lee H J, Shen W C, Lee H T
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
J Virol. 1992 Mar;66(3):1442-8. doi: 10.1128/JVI.66.3.1442-1448.1992.
Hz-1 viral RNA transcription was studied during productive and persistent infections. The RNAs were localized to 10- to 30-kb regions within the viral genome, and the timing of their expression was determined. During productive infections, we detected 101 virus-specific transcripts that could be grouped into three categories by time of appearance. At 2 h postinoculation (p.i.), a total of 34 virus-specific transcripts were detected. An additional 51 and 16 virus-specific transcripts appeared between 4 and 6 h p.i. and at 8 h p.i., respectively. After 8 h, no new transcripts were found. Under conditions of persistent infection, we detected only one viral persistency-associated transcript (PAT1). The region of the viral DNA which encodes PAT1 was cloned. During productive infections, three transcripts were derived from this region. Each had the same polarity as PAT1. One of them was of the same size as PAT1 and had similar, if not identical, 3' and 5' ends. This report provides detailed and very useful information concerning sequentially expressed transcripts of the Hz-1 baculovirus.
在增殖性感染和持续性感染期间对Hz - 1病毒RNA转录进行了研究。这些RNA定位于病毒基因组内10至30kb的区域，并确定了它们的表达时间。在增殖性感染期间，我们检测到101种病毒特异性转录本，根据出现时间可分为三类。接种后2小时（p.i.），共检测到34种病毒特异性转录本。另外分别在接种后4至6小时和8小时出现了51种和16种病毒特异性转录本。8小时后，未发现新的转录本。在持续性感染条件下，我们仅检测到一种病毒持续性相关转录本（PAT1）。编码PAT1的病毒DNA区域被克隆。在增殖性感染期间，该区域产生了三种转录本。每种转录本与PAT1具有相同的极性。其中一种与PAT1大小相同，并且具有相似（如果不是相同）的3'和5'末端。本报告提供了有关Hz - 1杆状病毒顺序表达转录本的详细且非常有用的信息。
