Kumar Vikas, Sahu N P, Pal A K, Kumar Shivendra
Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Seven Bungalow, Versova, Andheri (W), Mumbai 400061, Maharashtra, India.
Fish Shellfish Immunol. 2007 Aug;23(2):341-53. doi: 10.1016/j.fsi.2006.11.008. Epub 2006 Nov 30.
A 60 days experiment was conducted to study the effect of dietary gelatinized (G) and non-gelatinized (NG) starch on immunomodulation of Labeo rohita juveniles. Two hundred and thirty four juveniles (av. wt. 2.53+/-0.04) were randomly distributed in six treatment groups with each of three replicates. Six semi-purified diets containing NG and G corn starch, each at six levels of inclusion (0, 20, 40, 60, 80, 100) were prepared viz., T(1) (100% NG, 0% G starch), T(2) (80% NG, 20% G starch), T(3) (60% NG, 40% G starch), T(4) (40% NG, 60% G starch), T(5) (20% NG, 80% G starch) and T(6) (0% NG, 100% G starch). After a feeding period of 60 days, the juveniles were challenged with Aeromonas hydrophila to study their immunomodulation due to feeding of G and NG starch. RBC and haemoglobin content were significantly (P<0.05) reduced due to bacterial challenge, but dietary starch (G/NG starch) had no effect on it. G:NG starch ratio in the feed had significant effect on total leukocyte count during pre- and post-challenge periods. The leukocyte count concomitantly increased with the increased level of G starch in the diet. Highest albumin/globulin (A/G) ratio was recorded in T6 group (100% G starch) and lowest in T1 group (100% NG starch) group followed by T2 group both in pre- and post-challenge periods. NBT, lysozyme activity, total protein and globulin content were highest in T2 group (80% NG, 20% G starch) both in pre- and post-challenge periods. After challenge with A. hydrophila, the highest survival was recorded in T2 group, whereas lowest survival was recorded in T6 group. Conclusively high level of G starch was found to be immunosuppressive in Labeo rohita juveniles and NG:G starch ratio of 80:20 seems to be optimum for promoting growth and protecting immunity in L. rohita juveniles.
进行了一项为期60天的实验，以研究日粮中糊化（G）淀粉和未糊化（NG）淀粉对罗氏沼虾幼鱼免疫调节的影响。234尾幼鱼（平均体重2.53±0.04）被随机分配到六个处理组，每组三个重复。制备了六种含有NG和G玉米淀粉的半纯化日粮，每种淀粉的添加水平有六个（0、20、40、60、80、100），即T(1)（100%NG，0%G淀粉）、T(2)（80%NG，20%G淀粉）、T(3)（60%NG，40%G淀粉）、T(4)（40%NG，60%G淀粉）、T(5)（20%NG，80%G淀粉）和T(6)（0%NG，100%G淀粉）。在60天的投喂期后，用嗜水气单胞菌对幼鱼进行攻毒，以研究由于投喂G和NG淀粉对其免疫调节的影响。红细胞和血红蛋白含量因细菌攻毒而显著（P<0.05）降低，但日粮淀粉（G/NG淀粉）对其没有影响。饲料中G:NG淀粉比例在攻毒前后对白细胞总数有显著影响。白细胞计数随着日粮中G淀粉水平的增加而相应增加。在攻毒前后，T6组（100%G淀粉）的白蛋白/球蛋白（A/G）比值最高，T1组（100%NG淀粉）最低，其次是T2组。在攻毒前后，T2组（80%NG，20%G淀粉）的NBT、溶菌酶活性、总蛋白和球蛋白含量最高。用嗜水气单胞菌攻毒后，T2组的存活率最高，而T6组的存活率最低。结论是，高含量的G淀粉对罗氏沼虾幼鱼具有免疫抑制作用，NG:G淀粉比例为80:20似乎最有利于促进罗氏沼虾幼鱼的生长和保护其免疫力。
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