Pratheepa Vijayakumari, Sukumaran NatarajaPillai
Department of Aquaculture Biotechnology, Manonmaniam Sundaranar University, Sri Paramakalyani Centre for Environmental Sciences , Alwarkurchi, Tamil Nadu , India ; CIIMAR, Marine and Environmental Research Center, University of Porto , Rua dos Bragas, Porto , Portugal.
Department of Aquaculture Biotechnology, Manonmaniam Sundaranar University, Sri Paramakalyani Centre for Environmental Sciences , Alwarkurchi, Tamil Nadu , India.
PeerJ. 2014 Nov 13;2:e671. doi: 10.7717/peerj.671. eCollection 2014.
The main objective of the present study is to improve the immune power of Cyprinus carpio by using Euphorbia hirta plant leaf extract as immunostimulants. The haematological, immunological and enzymatic studies were conducted on the medicated fish infected with Aeromonas hydrophila pathogen. The results obtained from the haematological studies show that the RBC count, WBC count and haemoglobin content were increased in the infected fish at higher concentration of leaf extract. The feeds with leaf extract of Euphorbia hirta were able to stimulate the specific immune response by increasing the titre value of antibody. It was able to stimulate the antibody production only up to the 5th day, when fed with higher concentrations of (25 g and 50 g) plant leaf extract. The plant extract showed non-specific immune responses such as lysozyme activity, phagocytic ratio, NBT assay, etc. at higher concentration (50 g) and in the same concentration (50 g), the leaf extract of Euphorbia hirta significantly eliminated the pathogen in blood and kidney. It was observed that fish have survival percentage significantly at higher concentration (50 g) of Euphorbia hirta, when compared with the control. The obtained results are statistically significant at P < 0.05 and P < 0.01 levels. This research work suggests that the plant Euphorbia hirta has immunostimulant activity by stimulating both specific and non-specific immunity at higher concentrations.
本研究的主要目的是通过使用大戟叶提取物作为免疫刺激剂来提高鲤鱼的免疫力。对感染嗜水气单胞菌病原体的用药鱼进行了血液学、免疫学和酶学研究。血液学研究结果表明，在较高浓度的叶提取物作用下，感染鱼的红细胞计数、白细胞计数和血红蛋白含量均有所增加。含有大戟叶提取物的饲料能够通过提高抗体滴度值来刺激特异性免疫反应。当投喂较高浓度（25克和50克）的植物叶提取物时，它仅在第5天能够刺激抗体产生。植物提取物在较高浓度（50克）时表现出非特异性免疫反应，如溶菌酶活性、吞噬率、NBT试验等，并且在相同浓度（50克）下，大戟叶提取物能显著清除血液和肾脏中的病原体。观察到与对照组相比，在较高浓度（50克）的大戟叶作用下鱼的存活率显著提高。所获得的结果在P < 0.05和P < 0.01水平上具有统计学意义。这项研究工作表明，大戟属植物在较高浓度下通过刺激特异性和非特异性免疫具有免疫刺激活性。
