Choudhury Dharitri, Pal A K, Sahu N P, Kumar Shivendra, Das S S, Mukherjee S C
Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai, India.
Fish Shellfish Immunol. 2005 Sep;19(3):281-91. doi: 10.1016/j.fsi.2005.01.004.
A feeding trial was conducted for 60 days to delineate the effect of dietary ribonucleic acid or chitin on haematological parameters, phagocyte respiratory burst and resistance to Aeromonas hydrophila of Labeo rohita juveniles. One hundred and twenty-six (avg. wt. 13.40 +/- 0.17 g) juveniles were randomly distributed in six treatment groups, each one in three replicates. Six isonitrogenous (crude protein: 34.34-35.37%) and isocaloric (414-425 kcal 100 g(-1)) purified diets were prepared with different concentrations of either ribonucleic acid or chitin except the control group, viz., control, T1 (0.1% ribonucleic acid), T2 (0.2% ribonucleic acid), T3 (0.4% ribonucleic acid), T4 (25 mg chitin kg (-1)) and T5 (50 mg chitin kg (-1)). Weight gain %, specific growth rate, feed efficiency ratio, protein efficiency ratio did not vary significantly (P > 0.05) among the experimental groups. Haemoglobin content and total erythrocyte count were observed within the normal range and were not influenced by the dietary immunostimulants. Highest total leukocyte count was found in the T(3) group. The immunomodulatory effects of dietary immunostimulants were studied by using nitroblue tetrazolium (NBT) assay and serum parameters, namely total protein, albumin, globulin and A/G ratio. The respiratory burst activity (NBT) of blood phagocytes was highest in the T3 group followed by the T2 group, which varied significantly (P < 0.05) from other groups. Significantly (P < 0.05) higher total protein, globulin and lower A/G ratio was observed in the T(3) group. The relative percent survival after challenging with Aeromonas hydrophila was highest in the T3 group, compared to the control group, followed by the T2 group. The results indicate that dietary ribonucleic acid at 0.4% enhances phagocyte respiratory burst and protection of Labeo rohita juveniles to challenge by A. hydrophila.
进行了为期60天的饲养试验，以确定日粮中核糖核酸或几丁质对露斯塔野鲮幼鱼血液学参数、吞噬细胞呼吸爆发及对嗜水气单胞菌抵抗力的影响。126尾（平均体重13.40±0.17克）幼鱼随机分为6个处理组，每组3个重复。除对照组外，制备了6种等氮（粗蛋白：34.34 - 35.37%）和等热量（414 - 425千卡/100克）的纯化日粮，分别含有不同浓度的核糖核酸或几丁质，即对照组、T1（0.1%核糖核酸）、T2（0.2%核糖核酸）、T3（0.4%核糖核酸）、T4（25毫克几丁质/千克）和T5（50毫克几丁质/千克）。各实验组间的增重率、特定生长率、饲料效率比、蛋白质效率比无显著差异（P>0.05）。血红蛋白含量和红细胞总数在正常范围内，不受日粮免疫刺激剂的影响。T3组的白细胞总数最高。通过硝基蓝四氮唑（NBT）试验和血清参数，即总蛋白、白蛋白、球蛋白和A/G比值，研究了日粮免疫刺激剂的免疫调节作用。血液吞噬细胞的呼吸爆发活性（NBT）在T3组最高，其次是T2组，与其他组差异显著（P<0.05）。T3组的总蛋白、球蛋白显著（P<0.05）升高，A/G比值降低。与对照组相比，T3组经嗜水气单胞菌攻毒后的相对存活率最高，其次是T2组。结果表明，日粮中0.4%的核糖核酸可增强露斯塔野鲮幼鱼的吞噬细胞呼吸爆发及对嗜水气单胞菌攻毒的保护作用。
