College of Fisheries, Huazhong Agriculture University, Wuhan 430070, Hubei Province, China.
Fish Physiol Biochem. 2013 Apr;39(2):181-90. doi: 10.1007/s10695-012-9689-y. Epub 2012 Jul 12.
The effect of dietary intake of Coriolus versicolor Polysaccharides (CVP) on the hematological and biochemical indices of Allogynogenetic crucian carp (Carassius auratus gibelio) was investigated. Fish were fed CVP supplemented diets (0, 0.25, 0.5, 1.0, 2.0 or 4.0 g CVP kg(-1)) for 56 days. The RBC, WBC counts, hemoglobin content, ESR in blood and TP, ALT, AST, ALP, GLU, CHO, TG, and BUN in serum were measured on day 0, 14, 28, 42, and 56. After feeding of 56 days, fish were infected with Aeromonas hydrophila and mortalities were recorded. The results indicated that feeding crucian carp with suitable dose of CVP enhanced the RBC, WBC counts, hemoglobin and TP content, ALP activity, and decreased the ESR, ALT, AST, GLU, CHO, TG and BUN. There was no effect in fish at low dose (0.25 g kg(-1)). Unexpectedly, the higher CVP dose used here (2.0 and 4.0 g kg(-1)) has a negative effect in fish. The results of challenge experiment indicated that a moderate level of CVP in the diet (1.0 g kg(-1)) was the most effective to enhance the survival of fish after infected with A. hydrophila. In summary, the use of CVP, as dietary supplements, can improve the innate defense of crucian carp providing resistance to pathogens.
研究了杂色鲍多糖（CVP）的膳食摄入量对异育银鲫（Carassius auratus gibelio）血液学和生化指标的影响。鱼用添加 CVP 的饲料（0、0.25、0.5、1.0、2.0 或 4.0 g CVP kg(-1)) 喂养 56 天。在第 0、14、28、42 和 56 天测量 RBC、WBC 计数、血红蛋白含量、血液中的 ESR 以及血清中的 TP、ALT、AST、ALP、GLU、CHO、TG 和 BUN。喂养 56 天后，鱼被感染嗜水气单胞菌并记录死亡率。结果表明，用适量的 CVP 喂养鲫鱼可提高 RBC、WBC 计数、血红蛋白和 TP 含量、ALP 活性，并降低 ESR、ALT、AST、GLU、CHO、TG 和 BUN。低剂量（0.25 g kg(-1))对鱼没有影响。出乎意料的是，这里使用的更高 CVP 剂量（2.0 和 4.0 g kg(-1))对鱼有负面影响。挑战实验的结果表明，饮食中适量的 CVP（1.0 g kg(-1))是在感染嗜水气单胞菌后提高鱼存活率的最有效方法。总之，作为膳食补充剂，CVP 的使用可以提高鲫鱼的先天防御能力，提供对病原体的抵抗力。
