Schramm W, Pomerleau O F, Pomerleau C S, Grates H E
Reproductive Sciences Program, University of Michigan, Ann Arbor 48109.
Prev Med. 1992 Jan;21(1):63-73. doi: 10.1016/0091-7435(92)90006-4.
We have developed a device for the simplified collection of a prepurified sample of saliva in the mouth.
The device is based on the principle of an osmotic pump and accumulated about 1.2 ml of an ultrafiltrate of saliva within 8 min. We have investigated the ultrafiltrate for its utility as a biological medium in the evaluation of cigarette smoking status.
(a) In 58 matched samples from 13 subjects, the correlation coefficient for the cotinine concentration in the saliva and the ultrafiltrate was 0.95; (b) in matched plasma and ultrafiltrate samples from 27 smokers, the correlation coefficient for the cotinine concentrations was 0.96 with plasma containing 1.2 times the ultrafiltrate mean; (c) in a nonsmoker, elevated cotinine levels could be detected in the ultrafiltrate more than 24 hr after smoking 2 cigarettes, and the pattern of rise and decrease reflected that in whole saliva; and (d) in a habitual smoker; the mean cotinine concentration in the ultrafiltrate was 157 ng/ml (SD +/- 25.7 ng/ml) during a period of smoking 15 cigarettes per day and dropped to a mean of 47 ng/ml (SD +/- 10.5) when smoking was reduced to 5 cigarettes per day; after cessation of smoking, detectable concentrations of cotinine persisted for up to 5 days.
The device facilitated the aesthetic, noninvasive collection of a biological sample useful in the validation of smoking status.
我们研发了一种用于在口腔内简化收集预纯化唾液样本的装置。
该装置基于渗透泵原理，在8分钟内收集约1.2毫升唾液超滤液。我们研究了该超滤液作为评估吸烟状况的生物介质的效用。
（a）在来自13名受试者的58对匹配样本中，唾液和超滤液中可替宁浓度的相关系数为0.95；（b）在来自27名吸烟者的匹配血浆和超滤液样本中，可替宁浓度的相关系数为0.96，血浆中可替宁含量是超滤液平均值的1.2倍；（c）在一名不吸烟者中，吸两支烟24小时后，超滤液中可检测到可替宁水平升高，其升降模式与全唾液中的一致；（d）在一名习惯性吸烟者中，每天吸15支烟期间，超滤液中可替宁平均浓度为157纳克/毫升（标准差±25.7纳克/毫升），当吸烟量减至每天5支时，降至平均47纳克/毫升（标准差±10.5）；戒烟后，可检测到的可替宁浓度持续长达5天。
该装置有助于美观、无创地收集用于验证吸烟状况的生物样本。
