Schramm W, Annesley T M, Siegel G J, Sackellares J C, Smith R H
Reproductive Sciences Program, University of Michigan, Ann Arbor.
Ther Drug Monit. 1991 Sep;13(5):452-60. doi: 10.1097/00007691-199109000-00011.
We have introduced a method of collecting a prepurified sample of saliva in the mouth for the quantitative determination of phenytoin and carbamazepine. The patient places in the mouth an osmotic device that accumulates in greater than 8 min a volume of approximately 1.2 ml clear ultrafiltrate devoid of molecules greater than 12,000 daltons. The concentrations of phenytoin and carbamazepine in serum (total and free fractions), whole saliva, and the salivary ultrafiltrate from patients receiving anticonvulsant treatment were measured and correlated. The correlation coefficients r (p less than 0.001) for phenytoin were: total in serum and ultrafiltrate r = 0.92; free in serum and ultrafiltrate r = 0.93; whole saliva and ultrafiltrate r = 0.95. The correlation coefficients for carbamazepine were (in the same order) 0.90, 0.92, and 0.93. It is concluded that the use of an ultrafiltrate as a biological medium simplifies the diagnostic evaluation of free circulating phenytoin and carbamazepine concentrations for monitoring therapy.
我们介绍了一种在口腔中收集预纯化唾液样本的方法，用于苯妥英和卡马西平的定量测定。患者将一个渗透装置放入口中，该装置在8分钟以上的时间内积累约1.2毫升不含大于12,000道尔顿分子的清澈超滤液。测量并关联了接受抗惊厥治疗患者的血清（总分数和游离分数）、全唾液和唾液超滤液中苯妥英和卡马西平的浓度。苯妥英的相关系数r（p小于0.001）为：血清总分数与超滤液r = 0.92；血清游离分数与超滤液r = 0.93；全唾液与超滤液r = 0.95。卡马西平的相关系数（按相同顺序）为0.90、0.92和0.93。结论是，使用超滤液作为生物介质简化了用于监测治疗的游离循环苯妥英和卡马西平浓度的诊断评估。
