Dickson C, Puma J P, Nandi S
J Virol. 1975 Jan;17(1):275-82. doi: 10.1128/JVI.17.1.275-282.1976.
Mouse mammary tumor virus-producing cultures of mouse mammary tumor cells synthesize a viral-related polypeptide of molecular weight of 73,000 (gp 73) which is rapidly labeled during a short pulse but disappears during the chase concomitantly with the appearance of label in the virion glycoproteins gp 49 and gp 37.5/33.5. The addition of the protein synthesis-inhibitor cycloheximide to the chase medium has little effect on this conversion. Treatment of the proposed precursor with alpha-chymotrypsin leads to the formation of a polypeptide of molecular weight 49,000, similar to the major virion glycoprotein. A comparison of tryptic digest maps of the glycoproteins involved supports the hypothesis that both the viral glycoproteins gp 49 and gp 37.5/33.5 are derived from gp 73.
产生小鼠乳腺肿瘤病毒的小鼠乳腺肿瘤细胞培养物合成一种分子量为73,000的病毒相关多肽（gp 73），该多肽在短脉冲期间被快速标记，但在追踪期间消失，同时病毒粒子糖蛋白gp 49和gp 37.5/33.5中出现标记。在追踪培养基中添加蛋白质合成抑制剂环己酰亚胺对这种转化影响很小。用α-胰凝乳蛋白酶处理推测的前体导致形成分子量为49,000的多肽，类似于主要的病毒粒子糖蛋白。对所涉及糖蛋白的胰蛋白酶消化图谱的比较支持了这样的假设，即病毒糖蛋白gp 49和gp 37.5/33.5均源自gp 73。
