Dickson C, Puma J P, Nandi S
J Virol. 1975 Aug;16(2):250-8. doi: 10.1128/JVI.16.2.250-258.1975.
Mouse mammary tumor virus polypeptides were detected in the cytoplasm of mouse mammary tumor cell cultures using immunological precipitation techniques. The anti-mouse mammary tumor virus serum precipitated the major virion glycoproteins gp49 and gp37.5/33.5 and a viral-related nonvirion glycoprotein of 76,000 daltons. Subcellular fractionation studies revelaed that the cell-associated virion glycoproteins were present in the membrane fraction. Pulsechase experiments indicated that a viral-related nonvirion glycoprotein of 76,000 daltons may be a precursor to one or more of the virion glycoproteins.
使用免疫沉淀技术在小鼠乳腺肿瘤细胞培养物的细胞质中检测到小鼠乳腺肿瘤病毒多肽。抗小鼠乳腺肿瘤病毒血清沉淀出主要的病毒粒子糖蛋白gp49和gp37.5/33.5以及一种76000道尔顿的病毒相关非病毒粒子糖蛋白。亚细胞分级分离研究表明，细胞相关的病毒粒子糖蛋白存在于膜部分。脉冲追踪实验表明，一种76000道尔顿的病毒相关非病毒粒子糖蛋白可能是一种或多种病毒粒子糖蛋白的前体。
