Izumi H, Oyama H, Ozawa H
Jpn J Pharmacol. 1975 Aug;25(4):375-81. doi: 10.1254/jjp.25.375.
The effect of the boiled supernatant on synaptosomal adenyl cyclase activity of rat cerebral cortex was investigated. The boiled supernatant markedly increased the accumulation of cyclic AMP in synaptosomes when added to adenyl cyclase system by a mechanism presumably unrelated to inhibition of cyclic AMP phosphod iesterase or adenosine triphosphatase. Magnesium ion was required for synaptosomal adenyl cyclase activity and its stimulation by the boiled supernatant. The result discerned by doubl reciprocal plots showed an increase in Vmax value of adenyl cyclase by addition of the boiled supernatant without significantly altering the affinity for substrate. The enzyme activity was not stimulated by dopamine, histamine and serotonin in either the absence and presence of the boiled supernatant.
研究了煮沸上清液对大鼠大脑皮层突触体腺苷酸环化酶活性的影响。当将煮沸上清液添加到腺苷酸环化酶系统中时，它通过一种可能与抑制环磷酸腺苷磷酸二酯酶或三磷酸腺苷酶无关的机制，显著增加了突触体中环磷酸腺苷的积累。镁离子是突触体腺苷酸环化酶活性及其受煮沸上清液刺激所必需的。双倒数作图法得出的结果显示，添加煮沸上清液可使腺苷酸环化酶的Vmax值增加，而对底物的亲和力没有显著改变。无论有无煮沸上清液，多巴胺、组胺和5-羟色胺均不能刺激该酶活性。
