Izumi H, Ozawa H
Jpn J Pharmacol. 1975 Dec;25(6):709-18. doi: 10.1254/jjp.25.709.
A study was made of the effects of various agents on adenylate cyclase in synaptosomes in both the absence and presence of the boiled supernatant from rat cerebral cortex. The activity of adenylate cyclase in these preparations was inhibited by the sulfhydryl reactive agent p-chloromercuribenzoate. Sulfhydryl compounds such cysteine, glutathione and Coenzyme A stimulated the enzymic activity in both the absence and presence of the boiled supernatant. The chelating agent 1.2-bis-(2-dicarboxymethylaminoethoxy)ethane caused a stimulation of the enzymic activity with and without the presence of the boiled supernatant. Adenine nucleotide (adenine, adenosine, AMP and ADP), GTP, Pi and carbamylcholine seemed to have little effect. The stimulatory substance in the boiled supernatant was estimated to have a molecular weight in the range of 1,000-1,300.
研究了在不存在和存在大鼠大脑皮层煮沸上清液的情况下，各种试剂对突触体中腺苷酸环化酶的影响。这些制剂中腺苷酸环化酶的活性受到巯基反应剂对氯汞苯甲酸的抑制。半胱氨酸、谷胱甘肽和辅酶A等巯基化合物在不存在和存在煮沸上清液的情况下均刺激酶活性。螯合剂1,2-双-(2-二羧甲基氨基乙氧基)乙烷在有和没有煮沸上清液存在的情况下均能刺激酶活性。腺嘌呤核苷酸（腺嘌呤、腺苷、AMP和ADP）、GTP、Pi和氨甲酰胆碱似乎影响很小。煮沸上清液中的刺激物质估计分子量在1000-1300范围内。
