Kämpfer Peter, Scholz Holger, Huber Birgit, Thummes Kathrin, Busse Hans-Jürgen, Maas Elizabeth W, Falsen Enevold
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Bundeswehr Institute of Microbiology, D-80937 Munich, Germany.
Int J Syst Evol Microbiol. 2007 Apr;57(Pt 4):755-760. doi: 10.1099/ijs.0.64714-0.
A Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (strain CCUG 49584(T)), isolated from a seafood processing plant sample in New Zealand, was subjected to a polyphasic taxonomic study. On the basis of 16S rRNA and recA gene sequence similarities, the isolate was allocated to the genus Pseudochrobactrum. This was confirmed by fatty acid data (major fatty acids: C(18 : 1)omega7c and C(19 : 0) cyclo omega8c), a polar lipid profile exhibiting major characteristics of Pseudochrobactrum (phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine), quinone system Q-10 and a polyamine pattern with the predominant compounds spermidine and putrescine. DNA-DNA hybridization with the type strains of the two established species of Pseudochrobactrum and physiological and biochemical data clearly differentiated the isolate from established Pseudochrobactrum species. As a consequence, this organism represents a novel species, for which the name Pseudochrobactrum kiredjianiae sp. nov. is proposed, with the type strain CCUG 49584(T) (=CIP 109227(T)).
从新西兰一家海鲜加工厂的样本中分离出一株革兰氏阴性、杆状、氧化酶阳性、不形成芽孢、无运动性的细菌(菌株CCUG 49584(T)),对其进行了多相分类研究。基于16S rRNA和recA基因序列相似性,该分离株被归入假色杆菌属。脂肪酸数据(主要脂肪酸:C(18 : 1)ω7c和C(19 : 0)环ω8c)、显示假色杆菌主要特征的极性脂质谱(磷脂酰乙醇胺、磷脂酰单甲基乙醇胺、磷脂酰甘油、二磷脂酰甘油、磷脂酰胆碱)、醌系统Q-10以及以亚精胺和腐胺为主要化合物的多胺模式证实了这一点。与假色杆菌属两个已确立物种的模式菌株进行的DNA-DNA杂交以及生理生化数据清楚地将该分离株与已确立的假色杆菌物种区分开来。因此,该菌株代表一个新物种,为此提出名为Pseudochrobactrum kiredjianiae sp. nov.的新名称,模式菌株为CCUG 49584(T)(=CIP 109227(T))。