Dpto. de Microbiología y Parasitología, Instituto de Salud Tropical (ISTUN), Instituto de Investigación Sanitaria de Navarra (IdiSNA), Universidad de Navarra, Pamplona, Spain.
Laboratory of Experimental Biology and Pharmacology. Department of Biology, University of Medea, 26000 Medea, Algeria.
Int J Syst Evol Microbiol. 2022 Feb;72(2). doi: 10.1099/ijsem.0.005223.
Three Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile strains (C130915_07, C150915_16 and C150915_17) were isolated from lymph nodes of Algerian cows. On the basis of 16S rRNA gene and whole genome similarities, the isolates were almost identical and clearly grouped in the genus . This allocation was confirmed by the analysis of fatty acids (C, C, C, C and C) and of polar lipids (major components: phosphatidylethanolamine, ornithine-lipids, phosphatidylglycerol, cardiolipin and phosphatidylcholine, plus moderate amounts of phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine and other aminolipids). Genomic, physiological and biochemical data differentiated these isolates from previously described species in DNA relatedness, carbon assimilation pattern and growth temperature range. Thus, these organisms represent a novel species of the genus , for which the name sp. nov. is proposed (type strain C130915_07=CECT30232=LMG 32378).
三株革兰氏阴性、杆状、氧化酶阳性、不产芽孢、不运动的菌株(C130915_07、C150915_16 和 C150915_17)从阿尔及利亚奶牛的淋巴结中分离出来。基于 16S rRNA 基因和全基因组相似性,这些分离株几乎完全相同,并在属中明确分组。通过脂肪酸(C、C、C、C 和 C)和极性脂质(主要成分:磷脂酰乙醇胺、鸟氨酸脂、磷脂酰甘油、心磷脂和磷脂酰胆碱,加上适量的磷脂单甲基乙醇胺、磷脂二甲基乙醇胺和其他胺脂)分析,进一步证实了这一分类。基因组、生理和生化数据表明,这些分离株与先前描述的属内种在 DNA 相关性、碳同化模式和生长温度范围上存在差异。因此,这些生物代表了属的一个新种,提议使用 sp. nov. 命名(模式株 C130915_07=CECT30232=LMG 32378)。