Recombinant polypeptide antigen-based immunoglobulin G enzyme-linked immunosorbent assay for serodiagnosis of dengue.
作者信息
Dos Santos Flavia B, Nogueira Rita Maria R, Lima Monique R Q, De Simone Thatiane S, Schatzmayr Hermann G, Lemes Elezer M B, Harris Eva, Miagostovich Marize P
机构信息
Flavivirus Laboratory-Department of Virology, Oswaldo Cruz Institute/FIOCRUZ, Av Brasil, 4365, Manguinhos, Rio de Janeiro 21045-360, Brazil.
出版信息
Clin Vaccine Immunol. 2007 May;14(5):641-3. doi: 10.1128/CVI.00474-06. Epub 2007 Mar 28.
Abstract
We have developed an indirect enzyme-linked immunosorbent assay for detection of anti-dengue virus (DENV) immunoglobulin G antibodies using four recombinant DENV envelope polypeptides as antigens, which demonstrated a sensitivity of 89.4% and a specificity of 93.3%. These easily produced antigens are a feasible, cost-effective alternative for generating reagents for dengue serological tests.
摘要
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