Bentley D P, Blackmore J A
Department of Haematology, Llandough Hospital, Penarth, South Glamorgan, UK.
Br J Cancer. 1992 Feb;65(2):171-6. doi: 10.1038/bjc.1992.36.
The inhibition of 3H-thymidine incorporation into the DNA of mitogen-stimulated lymphocytes from patients with chronic lymphocytic leukaemia by chlorambucil was measured in vitro and the results related to clinical drug resistance. The assay proved to be both sensitive and specific showing a clear separation of those patients with responsive disease from those with disease resistant to treatment. There was evidence of primary drug resistance in untreated patients. In almost all patients who received treatment this led to increasing resistance to chlorambucil in vitro. The assay is predictive of clinical responsiveness and provides a potential means whereby new therapeutic agents and treatment modifiers may be investigated.
在体外测量了苯丁酸氮芥对慢性淋巴细胞白血病患者有丝分裂原刺激的淋巴细胞DNA中3H-胸腺嘧啶核苷掺入的抑制作用，并将结果与临床耐药性相关联。该测定方法被证明既敏感又特异，能清晰区分疾病有反应的患者和对治疗耐药的患者。在未经治疗的患者中有原发性耐药的证据。在几乎所有接受治疗的患者中，这导致体外对苯丁酸氮芥的耐药性增加。该测定方法可预测临床反应性，并提供了一种潜在手段，借此可研究新的治疗药物和治疗调节剂。
