Gras M, Crèche J, Chénieux J C, Rideau M
Laboratoire de Biologie Végétale, Faculté de Pharmacie, Tours, France.
Planta Med. 1982 Dec;46(4):231-5. doi: 10.1055/s-2007-971221.
The accumulation of two dihydrofuroquinoline alkaloids has been studied in seventeen strains of Choisya ternata (Rutaceae) initiated from the same tree. The contents of platydesminium and balfourodinium vary in heavy proportions. The effect of some factors (age of the culture, NAA or glucose concentrations of the medium, light intensity) has been studied in two of these strains, one with a low accumulative capacity, the other with a high accumulative capacity. These factors may in certain cases increase the alkaloid contents of both strains, but the induced variability is much lower than that existing naturally between the strains. The strain with a low content always remains inferior to that with a high content. This shows the importance of selecting strains with a high accumulative capacity before studying an optimal production medium.
对源自同一棵树的17个墨西哥橘(芸香科)菌株中二氢呋喃喹啉生物碱的积累情况进行了研究。普拉替定和巴尔弗罗定的含量差异很大。在其中两个菌株中研究了一些因素(培养物的年龄、培养基中萘乙酸或葡萄糖的浓度、光照强度)的影响,一个积累能力低,另一个积累能力高。在某些情况下,这些因素可能会增加两个菌株的生物碱含量,但诱导变异远低于菌株间自然存在的变异。含量低的菌株始终低于含量高的菌株。这表明在研究最佳生产培养基之前,选择积累能力高的菌株非常重要。