Cao Limin, Lin Hong, Mirsky Vladimir M
Aquatic Products Safety Laboratory, Ocean University of China, Qingdao 266003, PR China.
Anal Chim Acta. 2007 Apr 18;589(1):1-5. doi: 10.1016/j.aca.2007.02.034. Epub 2007 Feb 20.
A DNA-based surface plasmon resonance biosensor for enrofloxacin was developed. Heating denatured DNA immobilized on the gold-coated glass surface was exploited. The immobilization was performed by a layer-by-layer co-deposition with a cationic polymer. The sensor performance was tested with real biological probes. Direct and simple determination of enrofloxacin in milk samples was demonstrated. The sensor response obeys Langmuir binding isotherm being almost linear until about 20 microg mL(-1). The detection limit in milk samples was estimated to be 3 microg mL(-1).
开发了一种基于DNA的恩诺沙星表面等离子体共振生物传感器。利用加热变性的DNA固定在金涂层玻璃表面。通过与阳离子聚合物逐层共沉积进行固定。用实际生物探针测试了传感器性能。展示了直接、简单地测定牛奶样品中恩诺沙星的方法。传感器响应符合朗缪尔结合等温线，在约20μg mL⁻¹之前几乎呈线性。牛奶样品中的检测限估计为3μg mL⁻¹。
