Yanagida Akio, Yamakawa Yutaka, Noji Ryoko, Oda Ako, Shindo Heisaburo, Ito Yoichiro, Shibusawa Yoichi
Division of Structural Biology and Analytical Science, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
J Chromatogr A. 2007 Jun 1;1151(1-2):74-81. doi: 10.1016/j.chroma.2007.03.071. Epub 2007 Mar 23.
High-speed counter-current chromatography (HSCCC) using the three-phase solvent system n-hexane-methyl acetate-acetonitrile-water at a volume ratio of 4:4:3:4 was applied to the comprehensive separation of secondary metabolites in several natural product extracts. A wide variety of secondary metabolites in each natural product was effectively extracted with the three-phase solvent system, and the filtered extract was directly submitted to the HSCCC separation using the same three-phase system. In the HSCCC profiles of crude natural drugs listed in the Japanese Pharmacopoeia, several physiologically active compounds were clearly separated from other components in the extracts. The HSCCC profiles of several tea products, each manufactured by a different process, clearly showed their compositional difference in main compounds such as catechins, caffeine, and pigments. These HSCCC profiles also provide useful information about hydrophobic diversity of whole components present in each natural product.
使用体积比为4:4:3:4的正己烷-乙酸甲酯-乙腈-水三相溶剂系统的高速逆流色谱（HSCCC）被应用于几种天然产物提取物中次生代谢物的综合分离。每个天然产物中的多种次生代谢物都能被该三相溶剂系统有效萃取，过滤后的提取物直接使用相同的三相系统进行HSCCC分离。在《日本药局方》所列天然药物粗品的HSCCC图谱中，几种生理活性化合物与提取物中的其他成分被清晰分离。几种采用不同工艺生产的茶产品的HSCCC图谱清楚地显示了它们在儿茶素、咖啡因和色素等主要化合物组成上的差异。这些HSCCC图谱还提供了有关每种天然产物中所有成分疏水多样性的有用信息。
