Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Science, 52 Sanlihe Rd Xining 810001, P.R. China.
Savaid Medical School, University of the Chinese Academy of Sciences, 19B Yuquan Road, Beijing 100049, P.R. China.
J Chromatogr Sci. 2021 Jan 14;59(2):175-181. doi: 10.1093/chromsci/bmaa094.
Separation of natural compounds directly from the crude extract is a challenging work for traditional column chromatography. In the present study, an efficient method for separation of three main compounds from the crude extract of Dracocephalum tanguticum has been successfully established by high-speed counter-current chromatography (HSCCC). The crude extract was directly introduced into HSCCC by using dimethyl sulfoxide as cosolvent. Ethyl acetate/n-butyl alcohol/0.3% glacial acetic acid (4: 1: 5, v/v) system was used and three target compounds with purity higher than 80% were obtained. Preparative HPLC was used for further purification and three target compounds with purity higher than 98% were obtained. The compounds were identified as chlorogenic acid, pedaliin and pedaliin-6″-acetate.
从粗提物中直接分离天然化合物是传统柱层析的一项挑战性工作。本研究采用高速逆流色谱（HSCCC）成功建立了一种从唐古特青兰粗提取物中分离三种主要化合物的有效方法。粗提取物直接用二甲亚砜作为溶剂引入 HSCCC。采用乙酸乙酯/正丁醇/0.3%冰醋酸（4:1:5，v/v）体系，得到三种纯度高于 80%的目标化合物。制备 HPLC 进一步纯化，得到三种纯度高于 98%的目标化合物。这些化合物被鉴定为绿原酸、佩兰苷和佩兰苷-6'-乙酸酯。
