Ray Amitabha, Nkhata Katai J, Grande Joseph P, Cleary Margot P
Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN 55912, USA.
Cancer Lett. 2007 Aug 18;253(2):291-300. doi: 10.1016/j.canlet.2007.02.005. Epub 2007 Mar 30.
Leptin enhances proliferation of estrogen receptor (ER)-positive breast cancer cells in vitro. Here, we compared mammary tumor (MT) formation from ER-positive (MCF-7) and ER-negative (MDA-MB-231) breast cancer cells in athymic mice fed a High-Fat diet to elevate serum leptin. Neither body weight, diet or serum leptin levels impacted MT latency, burden or tumor grade. However, protein expression in mammary fat pads exhibited elevated PCNA and Cyclin D1 while in MTs, Ob-Rb, IGF-IR, Bcl-2, and Bax were lower in Low-Fat versus High-Fat mice. In conclusion, diet rather than serum leptin impacted breast cancer cell tumor metabolism.
瘦素在体外可增强雌激素受体（ER）阳性乳腺癌细胞的增殖。在此，我们比较了在喂食高脂饮食以提高血清瘦素水平的无胸腺小鼠中，ER阳性（MCF-7）和ER阴性（MDA-MB-231）乳腺癌细胞形成乳腺肿瘤（MT）的情况。体重、饮食或血清瘦素水平均未影响MT的潜伏期、负担或肿瘤分级。然而，低脂饮食组与高脂饮食组小鼠相比，乳腺脂肪垫中的蛋白质表达显示增殖细胞核抗原（PCNA）和细胞周期蛋白D1升高，而在MT中，瘦素受体b（Ob-Rb）、胰岛素样生长因子1受体（IGF-IR）、B细胞淋巴瘤-2（Bcl-2）和Bax较低。总之，饮食而非血清瘦素影响乳腺癌细胞的肿瘤代谢。
