Bonorden Melissa J L, Grossmann Michael E, Ewing Sarah A, Rogozina Olga P, Ray Amitabha, Nkhata Katai J, Liao D Joshua, Grande Joseph P, Cleary Margot P
The Hormel Institute, University of Minnesota, Austin, MN 55912, USA.
Prostate Cancer. 2012;2012:543970. doi: 10.1155/2012/543970. Epub 2012 Dec 4.
To clarify effects of diet and body weight on prostate cancer development, three studies were undertaken using the TRAMP mouse model of this disease. In the first experiment, obesity was induced by injection of gold thioglucose (GTG). Age of prostate tumor detection (33 wk) and death (43 wk) was not significantly different among the groups. In the second study, TRAMP-C2 cells were injected into syngeneic C57BL6 mice and tumor progression was evaluated in mice fed either high-fat or low-fat diets. The high fat fed mice had larger tumors than did the low-fat fed mice. In the third study, tumor development was followed in TRAMP mice fed a high fat diet from 6 weeks of age. There were no significant effects of body weight status or diet on tumor development among the groups. When the tumors were examined for the neuroendocrine marker synaptophysin, there was no correlation with either body weight or diet. However, there was a significant correlation of the expression of synaptophysin with earlier age to tumor detection and death. In summary, TRAMP-C2 cells grew faster when the mice were fed a high-fat diet. Further synaptophysin may be a marker of poor prognosis independent of weight and diet.
为阐明饮食和体重对前列腺癌发展的影响，利用该疾病的TRAMP小鼠模型进行了三项研究。在第一个实验中，通过注射金硫葡萄糖（GTG）诱导肥胖。各实验组之间前列腺肿瘤检测年龄（约33周）和死亡年龄（约43周）无显著差异。在第二项研究中，将TRAMP - C2细胞注射到同基因C57BL6小鼠体内，并对喂食高脂或低脂饮食的小鼠的肿瘤进展进行评估。高脂饮食喂养的小鼠肿瘤比低脂饮食喂养的小鼠大。在第三项研究中，对6周龄开始喂食高脂饮食的TRAMP小鼠的肿瘤发展进行跟踪。各实验组之间体重状况或饮食对肿瘤发展均无显著影响。当检测肿瘤的神经内分泌标志物突触素时，其与体重或饮食均无相关性。然而，突触素的表达与较早的肿瘤检测年龄和死亡年龄存在显著相关性。总之，当小鼠喂食高脂饮食时，TRAMP - C2细胞生长更快。此外，突触素可能是一个与体重和饮食无关的预后不良标志物。
