Robins S P, Bailey A J
Biochem J. 1977 May 1;163(2):339-46. doi: 10.1042/bj1630339.
Reduction of tissues with sodium cyanoborohydride at pH7.4 gave results identical with those obtained by KBH4 treatment. On reduction with sodium cyanoborohydride at pH 4.4, however, a previously undetected basic compound was formed and was identified by mass spectrometry and chemical degradation techniques as dihydrohydroxymerodesmosine. Histidino-hydroxymerodesmosine was not present, and further analysis confirmed that reduced aldol, a mojor product of reduction with KBH4 at the lower pH, was also absent. These results, together with an analysis of the time course of the reduction, support previous assertions that histidino-hydroxymerodesmosine is an artifact [robins *Bailey (1973) Biochem. J. 135, 657-665] and suggests that the non-reduced form of hydroxymerodesmosine probably does not constitute a major intermolecular bond in vivo.
在pH7.4条件下用氰基硼氢化钠还原组织，得到的结果与用KBH4处理得到的结果相同。然而，在pH 4.4条件下用氰基硼氢化钠还原时，会形成一种先前未检测到的碱性化合物，通过质谱和化学降解技术鉴定为二氢羟基异二联赖氨酸。不存在组氨酸-羟基异二联赖氨酸，进一步分析证实，在较低pH值下用KBH4还原的主要产物还原醛醇也不存在。这些结果，连同对还原时间进程的分析，支持了先前关于组氨酸-羟基异二联赖氨酸是一种假象的论断[罗宾斯*贝利（1973年）《生物化学杂志》135卷，657 - 665页]，并表明羟基异二联赖氨酸的未还原形式在体内可能不构成主要的分子间键。
