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Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
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Post-crystallization treatments for improving diffraction quality of protein crystals.用于提高蛋白质晶体衍射质量的结晶后处理方法。
Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1173-80. doi: 10.1107/S0907444905019451. Epub 2005 Aug 16.
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Extension of the diffraction resolution of crystals.晶体衍射分辨率的扩展
Acta Crystallogr D Biol Crystallogr. 1994 Jul 1;50(Pt 4):563-8. doi: 10.1107/S0907444994001976.
4
Crystal structure of a prokaryotic replication initiator protein bound to DNA at 2.6 A resolution.原核生物复制起始蛋白与DNA结合的晶体结构，分辨率为2.6埃。
EMBO J. 1999 Sep 1;18(17):4597-607. doi: 10.1093/emboj/18.17.4597.
5
Crystallization and preliminary X-ray diffraction studies of a replication initiator protein (RepE54) of the mini-F plasmid complexed with iteron DNA.与重复序列DNA复合的微小F质粒复制起始蛋白（RepE54）的结晶及初步X射线衍射研究。
J Biochem. 1999 Jan;125(1):24-6. doi: 10.1093/oxfordjournals.jbchem.a022262.
6
Macromolecular crystal annealing: overcoming increased mosaicity associated with cryocrystallography.大分子晶体退火：克服与低温晶体学相关的镶嵌性增加问题。
Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):622-8. doi: 10.1107/s0907444997019008.
7
The central region of RepE initiator protein of mini-F plasmid plays a crucial role in dimerization required for negative replication control.微小F质粒的RepE起始蛋白的中央区域在负向复制控制所需的二聚化过程中起着关键作用。
J Mol Biol. 1997 Nov 21;274(1):27-38. doi: 10.1006/jmbi.1997.1373.
8
The localized melting of mini-F origin by the combined action of the mini-F initiator protein (RepE) and HU and DnaA of Escherichia coli.通过大肠杆菌的微小F起始蛋白（RepE）以及HU和DnaA的联合作用实现微小F原点的局部解链。
Mol Gen Genet. 1996 Nov 27;253(1-2):42-9. doi: 10.1007/s004380050294.
9
Replication initiator protein RepE of mini-F plasmid: functional differentiation between monomers (initiator) and dimers (autogenous repressor).微小F质粒的复制起始蛋白RepE：单体（起始蛋白）与二聚体（自体阻遏物）之间的功能分化
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3839-43. doi: 10.1073/pnas.91.9.3839.
10
DNA-binding domain of the RepE initiator protein of mini-F plasmid: involvement of the carboxyl-terminal region.微小F质粒RepE起始蛋白的DNA结合结构域：羧基末端区域的作用
J Bacteriol. 1995 Apr;177(8):1994-2001. doi: 10.1128/jb.177.8.1994-2001.1995.

F 质粒 RepE 的表达与纯化及其与操纵子 DNA 复合物的初步 X 射线晶体学研究。

Expression and purification of F-plasmid RepE and preliminary X-ray crystallographic study of its complex with operator DNA.

作者信息

Nakamura Akira, Wada Chieko, Miki Kunio

机构信息

Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Apr 1;63(Pt 4):346-9. doi: 10.1107/S1744309107012894. Epub 2007 Mar 30.

DOI:10.1107/S1744309107012894
PMID:17401213
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2330221/
Abstract

The replication initiator factor RepE of the F plasmid in Escherichia coli is an essential protein that stringently regulates the F-plasmid copy number. The RepE protein has a dual function: its monomer functions as a replication initiator, while its dimer acts as a transcriptional repressor of the repE gene. The wild-type dimeric RepE protein was expressed as an N-terminal histidine-tagged protein, purified under native conditions with a high salt concentration and crystallized in complex with the repE operator DNA using the sitting-drop vapour-diffusion technique. The crystals diffracted to a resolution of 3.14 A after the application of dehydration and crystal annealing and belong to space group P2(1), with unit-cell parameters a = 60.73, b = 99.32, c = 95.00 A, beta = 108.55 degrees.

摘要

大肠杆菌中F质粒的复制起始因子RepE是一种必需蛋白，它严格调控F质粒的拷贝数。RepE蛋白具有双重功能：其单体作为复制起始因子，而其二聚体作为repE基因的转录抑制因子。野生型二聚体RepE蛋白表达为N端带组氨酸标签的蛋白，在高盐浓度的天然条件下纯化，并使用坐滴气相扩散技术与repE操纵子DNA形成复合物结晶。在进行脱水和晶体退火处理后，晶体的衍射分辨率达到3.14 Å，属于空间群P2(1)，晶胞参数为a = 60.73、b = 99.32、c = 95.00 Å，β = 108.55°。