自校准黏度探头：设计与亚细胞定位。
Self-calibrating viscosity probes: design and subcellular localization.
机构信息
Department of Chemistry & Biochemistry, University of California, San Diego, 9500 Gilman Drive, MC: 0358, La Jolla, CA 92093-0358, USA.
出版信息
Bioorg Med Chem. 2012 Jul 15;20(14):4443-50. doi: 10.1016/j.bmc.2012.05.026. Epub 2012 May 19.
Abstract
We describe the design, synthesis and fluorescence profiles of new self-calibrating viscosity dyes in which a coumarin (reference fluorophore) has been covalently linked with a molecular rotor (viscosity sensor). Characterization of their fluorescence properties was made with separate excitation of the units and through resonance energy transfer from the reference to the sensor dye. We have modified the linker and the substitution of the rotor in order to change the hydrophilicity of these probes thereby altering their subcellular localization. For instance, hydrophilic dye 12 shows a homogeneous distribution inside the cell and represents a suitable probe for viscosity measurements in the cytoplasm.
摘要
我们描述了新型自校准粘度染料的设计、合成和荧光特性，其中香豆素（参比荧光团）与分子转子（粘度传感器）共价连接。通过分别激发单元和通过从参比染料到传感器染料的共振能量转移来对其荧光性质进行了表征。我们已经修饰了连接体并改变了转子的取代基，从而改变了这些探针的亲水性，从而改变了它们的亚细胞定位。例如，亲水性染料 12 在细胞内均匀分布，是测量细胞质粘度的合适探针。
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