Sueda Jun, Fukuchi Toshio, Usumoto Nari, Okuno Takashi, Arai Mikki, Hirose Tatsuo
The Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts, USA.
Jpn J Ophthalmol. 2007 Mar-Apr;51(2):89-95. doi: 10.1007/s10384-006-0405-2. Epub 2007 Apr 6.
To evaluate the safety and efficacy of newly developed hydrogel glue to treat rhegmatogenous retinal detachments in in vitro and in vivo studies.
In the in vitro study, the solid hydrogel glue was soaked in a balanced salt solution (BSS). The pH was measured periodically, and the dissolution time was recorded. In the in vivo study in six albino rabbits, 0.1 ml of hydrogel glue was injected into the right vitreous cavity, and 0.1 ml BSS was injected into the left vitreous, as the control. Clinical, electroretinography (ERG) and histological examinations were performed. Retinal detachment with a retinal hole was created in 12 albino rabbits after vitrectomy. After fluid-air exchange, the hydrogel glue was applied to the hole in nine rabbits; three other rabbits were used as controls. Clinical and histological examinations were performed.
The pH ranged from 6.65 to 8.14. The glue remained solid in BSS for 7 weeks. The glue injection did not induce inflammation. There was no significant difference between the study and control eyes in the ERG amplitude or the implicit times of the a and b waves. No significant histological abnormality was detected. The retina was reattached with glue in three of nine eyes. The histological examination showed glue under the retina.
Hydrogel glue, which seemed to be minimally toxic to the eye, can be used to patch retinal breaks. However, methods to mix a small amount of each solution completely and a more effective intraocular delivery system for the glue are needed.
在体外和体内研究中评估新开发的水凝胶胶水治疗孔源性视网膜脱离的安全性和有效性。
在体外研究中，将固体水凝胶胶水浸泡在平衡盐溶液（BSS）中。定期测量pH值，并记录溶解时间。在对六只白化兔进行的体内研究中，向右侧玻璃体腔注射0.1 ml水凝胶胶水，向左侧玻璃体注射0.1 ml BSS作为对照。进行临床、视网膜电图（ERG）和组织学检查。在12只白化兔玻璃体切除术后制造带有视网膜裂孔的视网膜脱离。在液气交换后，对9只兔子的裂孔应用水凝胶胶水；另外3只兔子作为对照。进行临床和组织学检查。
pH值范围为6.65至8.14。胶水在BSS中保持固态7周。胶水注射未诱发炎症。研究组和对照组眼睛在ERG振幅或a波和b波的隐含时间方面无显著差异。未检测到明显的组织学异常。9只眼中有3只眼的视网膜用胶水重新附着。组织学检查显示视网膜下有胶水。
水凝胶胶水似乎对眼睛毒性极小，可用于修补视网膜裂孔。然而，需要将每种溶液少量完全混合的方法以及更有效的胶水眼内递送系统。
