Ravindranath N, Ramesh V, Krishnamurthy H N, Rao A J, Moudgal R N
Indian Institute of Science, Bangalore.
Fertil Steril. 1992 Mar;57(3):671-6. doi: 10.1016/s0015-0282(16)54919-x.
To study the efficacy of long-term buserelin acetate infusion to desensitize pituitary and block testicular function in adult male monkeys (Macaca radiata).
Proven fertile male monkeys exhibiting normal testicular function.
Each of the control (n = 5) and experimental monkeys (n = 10) received a fresh miniosmotic pump every 21 days, whereas pumps in controls delivered vehicle of experimentals released 50 micrograms buserelin acetate every 24 hours. On day 170 (renewed every 60 days) a silastic capsule containing crystalline testosterone (T) was implanted in the experimental monkeys. At the end of 3 years, treatment was stopped, and recovery of testicular function and fertility monitored.
(1) Treatment resulted in marked reduction of nocturnal but not basal serum T; (2) the pituitary remained desensitized to buserelin acetate throughout the 3-year period; (3) animals were largely azoospermic with occasional oligospermia exhibited by two monkeys; and (4) withdrawal of treatment restored testicular function, with 70% of animals regaining fertility.
Long-term infertility (but restorable) can be induced in male monkeys by constant infusion of buserelin acetate and T.
研究长期输注醋酸布舍瑞林对成年雄性恒河猴垂体脱敏及阻断睾丸功能的疗效。
经证实具有生育能力且睾丸功能正常的雄性恒河猴。
对照组（n = 5）和实验组猴子（n = 10）每21天更换一个新的微量渗透泵，对照组的泵输送赋形剂，实验组的泵每24小时释放50微克醋酸布舍瑞林。在第170天（每60天更换一次），给实验组猴子植入一个含有结晶睾酮（T）的硅橡胶胶囊。3年后，停止治疗，并监测睾丸功能和生育能力的恢复情况。
（1）治疗导致夜间血清睾酮显著降低，但基础血清睾酮未降低；（2）在整个3年期间，垂体对醋酸布舍瑞林仍处于脱敏状态；（3）动物大多无精子，两只猴子偶尔出现少精子症；（4）停止治疗后恢复了睾丸功能，70%的动物恢复了生育能力。
持续输注醋酸布舍瑞林和睾酮可诱导雄性猴子长期不育（但可恢复）。
