Tashiro K, Tsuru M
No Shinkei Geka. 1975 Apr;3(4):323-7.
The rapid histological diagnosis of neurosurgical biopsy material is of prime importance for the neurosurgeon. Cryostat-cut sections of fresh frozen tissues have been used in our department. The surgical materials are rapidly frozen at - 150 degrees C, and serial sections, 10 microns thick, are cut in a cryostat at -18 degrees to -20 degrees C. The sections are adhered to cover glasses and stained with Metachrome solution (a mixture of 1% Azure A and 0.5% Erie Garnet B). The rapid and accurate diagnosis can be obtained within 5 minutes. The advantage of cryostat-cut frozen section is the preservation of cell density and tissue architecture, which is most important in the diagnosis of neurosurgical materials, especially in case of diffusely infiltrating glioma. The previously reported methods, such as smear technique and frozen sections by using freezing microtome are also reviewed and discussed.
神经外科活检材料的快速组织学诊断对神经外科医生至关重要。我们科室采用新鲜冷冻组织的低温恒温器切片。手术材料在-150℃迅速冷冻，然后在-18℃至-20℃的低温恒温器中切成10微米厚的连续切片。切片粘附在盖玻片上，用异染溶液（1%天青A和0.5%伊利石榴石B的混合物）染色。5分钟内即可获得快速准确的诊断。低温恒温器切片冷冻切片的优点是保留细胞密度和组织结构，这在神经外科材料的诊断中最为重要，尤其是在弥漫性浸润性胶质瘤的情况下。还对先前报道的方法，如涂片技术和使用冷冻切片机的冷冻切片进行了回顾和讨论。
