Gutiérrez-Ruiz Ma Concepción, Gómez-Quiroz Luis Enrique
Departamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, México, DF, México.
Liver Int. 2007 May;27(4):434-9. doi: 10.1111/j.1478-3231.2007.01469.x.
Hepatic stellate cells (HSC) are the principal fibrogenic cell type in the liver. Progress in understanding the cellular and molecular basis for the development and progression of liver fibrosis could be possible by the development of methods to isolate HSC from rodents and human liver. Growth of stellate cells on plastic led to a phenotypic response known as activation, which paralleled closely the response of these cells to injury in vivo. Actually, much of the current knowledge of stellate cell behaviour has been gained through primary culture studies, particularly from rats. Also, different laboratories that have established hepatic stellate cell lines from rats and humans have provided a stable and unlimited source of cells that express specific functions, making them suitable for culture-based studies of hepatic fibrosis. From these in vitro models grew a large body of information characterizing stellate cell activation, cytokine signalling, intracellular pathways regulating liver fibrogenesis, production of extracellular matrix proteins and development of antifibrotic drugs.
肝星状细胞（HSC）是肝脏中主要的纤维化细胞类型。通过开发从啮齿动物和人类肝脏中分离肝星状细胞的方法，有可能在理解肝纤维化发生和进展的细胞及分子基础方面取得进展。星状细胞在塑料表面生长会导致一种称为激活的表型反应，这与这些细胞在体内对损伤的反应密切相似。实际上，目前关于星状细胞行为的许多知识都是通过原代培养研究获得的，特别是来自大鼠的研究。此外，不同实验室从大鼠和人类建立的肝星状细胞系提供了表达特定功能的稳定且无限的细胞来源，使其适用于基于培养的肝纤维化研究。从这些体外模型中产生了大量关于星状细胞激活、细胞因子信号传导、调节肝纤维化的细胞内途径、细胞外基质蛋白的产生以及抗纤维化药物开发的信息。
