Faculty of Pharmaceutical Sciences, Kyushu University, Japan.
Planta Med. 1983 Feb;47(2):103-5. doi: 10.1055/s-2007-969963.
Rapid and simple regeneration of Pinellia ternata has been achieved by tissue culture. The addition of 2,4-dichlorophenoxy acetic acid (2,4-D; 0.5 ppm) and kinetin (1 ppm) stimulated production of a maximum number of regenerated plantlets, while callus formation was induced by concentrations of 2,4-D higher than 1 ppm. alpha-Naphthaleneacetic acid (NAA) was also favourable for regeneration but was less effective than 2,4-D. Growth with indole-3-acetic acid (IAA) and kinetin favoured callus formation in general, but the rate of callus growth was extremely slow. These regenerated plantlets flowered true to their parental strains when transferred to soil and cultivated for 4.5 months.
通过组织培养，实现了半夏的快速、简单再生。添加 2,4-二氯苯氧乙酸（2,4-D；0.5 ppm）和激动素（1 ppm）刺激了最大数量的再生植物的产生，而愈伤组织的形成则是由高于 1 ppm 的 2,4-D 浓度诱导的。α-萘乙酸（NAA）也有利于再生，但效果不如 2,4-D。吲哚-3-乙酸（IAA）和激动素的生长通常有利于愈伤组织的形成，但愈伤组织的生长速度非常缓慢。这些再生的植物在转移到土壤中并培养 4.5 个月后，表现出与其亲本品种一致的开花特征。
