Faculty of Pharmaceutical Sciences, Kyushu University, Japan.
Planta Med. 1983 Sep;49(9):14-6. doi: 10.1055/s-2007-969800.
In M URASHIGE and S KOOG'S liquid medium supplemented with 0.25 ppm 2,4-dichlorophenoxyacetic acid(2,4-D) a tuber segment reached a fresh weight multiplication ratio of 200 when grown for 4 weeks at a rotation speed of 60 rev/min, at 25 degrees and in continuous light. The growth ability was maintained for 5 generations. Addition of 0.5 ppm 2,4-D and 1 ppm kinetin stimulated production of a maximum number of regenerated plantlets from the tissue mass segment. The regeneration ability was also estimated. It is theoretically possible to obtain more than 4 x 10 (23) plants from a single tuber in a year by this clonal propagation technique.
在 M URASHIGE 和 S KOOG 的液体培养基中添加 0.25ppm2,4-二氯苯氧乙酸（2,4-D），在转速为 60 转/分钟、25℃和连续光照下，薯块培养 4 周时达到鲜重倍增比 200。这种生长能力可维持 5 代。添加 0.5ppm2,4-D 和 1ppm 激动素可刺激组织质量段再生出最多数量的再生小植株。再生能力也得到了评估。通过这种克隆繁殖技术，理论上可以从单个薯块中获得超过 4 x 10 (23) 株植物。
