Liang Chun-Chi, Park Ann Y, Guan Jun-Lin
Division of Molecular Medicine and Genetics and Cell and Developmental Biology, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
Nat Protoc. 2007;2(2):329-33. doi: 10.1038/nprot.2007.30.
The in vitro scratch assay is an easy, low-cost and well-developed method to measure cell migration in vitro. The basic steps involve creating a "scratch" in a cell monolayer, capturing the images at the beginning and at regular intervals during cell migration to close the scratch, and comparing the images to quantify the migration rate of the cells. Compared to other methods, the in vitro scratch assay is particularly suitable for studies on the effects of cell-matrix and cell-cell interactions on cell migration, mimic cell migration during wound healing in vivo and are compatible with imaging of live cells during migration to monitor intracellular events if desired. Besides monitoring migration of homogenous cell populations, this method has also been adopted to measure migration of individual cells in the leading edge of the scratch. Not taking into account the time for transfection of cells, in vitro scratch assay per se usually takes from several hours to overnight.
体外划痕试验是一种简便、低成本且成熟的体外测量细胞迁移的方法。基本步骤包括在细胞单层上制造一个“划痕”，在细胞迁移以闭合划痕的开始阶段和定期时间点采集图像，并比较这些图像以量化细胞的迁移速率。与其他方法相比，体外划痕试验特别适用于研究细胞-基质和细胞-细胞相互作用对细胞迁移的影响，模拟体内伤口愈合过程中的细胞迁移，并且如果需要的话，在迁移过程中与活细胞成像兼容以监测细胞内事件。除了监测同质细胞群体的迁移外，该方法还被用于测量划痕前沿单个细胞的迁移。不考虑细胞转染时间，体外划痕试验本身通常需要数小时至过夜时间。
