Chong Kwok Feng, Loh Kian Ping, Vedula S R K, Lim Chwee Teck, Sternschulte Hadwig, Steinmüller Doris, Sheu Fwu-Shan, Zhong Yu Lin
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543.
Langmuir. 2007 May 8;23(10):5615-21. doi: 10.1021/la070037y. Epub 2007 Apr 4.
The biocompatibility of diamond was investigated with a view toward correlating surface chemistry and topography with cellular adhesion and growth. The adhesion properties of normal human dermal fibroblast (NHDF) cells on microcrystalline and ultrananocrystalline diamond (UNCD) surfaces were measured using atomic force microscopy. Cell adhesion forces increased by several times on the hydrogenated diamond surfaces after UV irradiation of the surfaces in air or after functionalization with undecylenic acid. A direct correlation between initial cell adhesion forces and the subsequent cell growth was observed. Cell adhesion forces were observed to be strongest on UV-treated UNCD, and cell growth experiments showed that UNCD was intrinsically more biocompatible than microcrystalline diamond surfaces. The surface carboxylic acid groups on the functionalized diamond surface provide tethering sites for laminin to support the growth of neuron cells. Finally, using capillary injection, a surface gradient of polyethylene glycol could be assembled on top of the diamond surface for the construction of a cell gradient.
为了将表面化学和形貌与细胞粘附及生长联系起来，对金刚石的生物相容性进行了研究。使用原子力显微镜测量了正常人皮肤成纤维细胞（NHDF）在微晶和超纳米晶金刚石（UNCD）表面的粘附特性。在空气中对表面进行紫外线照射后或用十一碳烯酸功能化后，氢化金刚石表面上的细胞粘附力增加了几倍。观察到初始细胞粘附力与随后的细胞生长之间存在直接相关性。在紫外线处理过的UNCD上观察到细胞粘附力最强，细胞生长实验表明，UNCD本质上比微晶金刚石表面具有更高的生物相容性。功能化金刚石表面上的表面羧酸基团为层粘连蛋白提供了拴系位点，以支持神经元细胞的生长。最后，使用毛细管注射，可以在金刚石表面顶部组装聚乙二醇的表面梯度，以构建细胞梯度。
