Hsia Chu Chieh, Chizhikov Vladimir E, Yang Amy X, Selvapandiyan Angamuthu, Hewlett Indira, Duncan Robert, Puri Raj K, Nakhasi Hira L, Kaplan Gerardo G
Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Review, Food and Drug Administration, Bethesda, MD 20892, USA.
Biochem Biophys Res Commun. 2007 May 18;356(4):1017-23. doi: 10.1016/j.bbrc.2007.03.087. Epub 2007 Mar 26.
Hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type-1 (HIV-1) are transfusion-transmitted human pathogens that have a major impact on blood safety and public health worldwide. We developed a microarray multiplex assay for the simultaneous detection and discrimination of these three viruses. The microarray consists of 16 oligonucleotide probes, immobilized on a silylated glass slide. Amplicons from multiplex PCR were labeled with Cy-5 and hybridized to the microarray. The assay detected 1 International Unit (IU), 10 IU, 20 IU of HBV, HCV, and HIV-1, respectively, in a single multiplex reaction. The assay also detected and discriminated the presence of two or three of these viruses in a single sample. Our data represent a proof-of-concept for the possible use of highly sensitive multiplex microarray assay to screen and confirm the presence of these viruses in blood donors and patients.
乙型肝炎病毒（HBV）、丙型肝炎病毒（HCV）和1型人类免疫缺陷病毒（HIV-1）是经输血传播的人类病原体，对全球血液安全和公共卫生有重大影响。我们开发了一种微阵列多重检测方法，用于同时检测和区分这三种病毒。该微阵列由固定在硅烷化载玻片上的16个寡核苷酸探针组成。多重PCR扩增产物用Cy-5标记，并与微阵列杂交。该检测方法在单次多重反应中分别检测到1国际单位（IU）、10 IU、20 IU的HBV、HCV和HIV-1。该检测方法还能在单个样本中检测并区分这三种病毒中的两种或三种的存在情况。我们的数据为使用高灵敏度多重微阵列检测方法筛查和确认献血者和患者中这些病毒的存在提供了概念验证。
