Someya Nobutaka, Tsuchiya Kenichi, Yoshida Takanobu, Noguchi Masako T, Akutsu Katsumi, Sawada Hiroyuki
National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan.
Biocontrol Sci. 2007 Mar;12(1):1-6. doi: 10.4265/bio.12.1.
The antifungal compound 2,4-diacetylphloroglucinol-producing bacterium, Pseudomonas fluorescens strain LRB3W1, inhibits the growth of Fusarium oxysporum f. sp. lycopersici, and controls Fusarium wilt of tomato caused by F. oxysporum f. sp. lycopersici. On the other hand, Serratia marcescens strain B2, which produces cell wall-degrading enzyme chitinases, did not inhibit fungal growth and the suppressive effect of strain B2 against tomato Fusarium wilt was less than that of strain LRB3W1. Combined inoculation of strain LRB3W1 with strain B2 was more effective than treatment with strain LRB3W1 alone. When 2,4-diacetylphloroglucinol and the chitinolytic enzymes were applied in combination, a synergistic inhibitory effect against the pathogen was observed. It was possible that bacteria which produce cell wall-degrading enzymes enhanced the biocontrol effect of the antibiotic-producing bacterium against tomato Fusarium wilt.
产抗真菌化合物2,4-二乙酰间苯三酚的荧光假单胞菌LRB3W1菌株能够抑制番茄枯萎病菌的生长，并防治由番茄枯萎病菌引起的番茄枯萎病。另一方面，产细胞壁降解酶几丁质酶的粘质沙雷氏菌B2菌株不抑制真菌生长，且B2菌株对番茄枯萎病的抑制效果低于LRB3W1菌株。LRB3W1菌株与B2菌株联合接种比单独使用LRB3W1菌株处理更有效。当将2,4-二乙酰间苯三酚和几丁质分解酶联合应用时，观察到对病原菌有协同抑制作用。产细胞壁降解酶的细菌有可能增强了产抗生素细菌对番茄枯萎病的生防效果。
