Prabhukarthikeyan Rathinam, Saravanakumar Duraisamy, Raguchander Thiruvengadam
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, India.
Pest Manag Sci. 2014 Nov;70(11):1742-50. doi: 10.1002/ps.3719. Epub 2014 Feb 17.
Most of the approaches for biocontrol of pests and diseases have used a single biocontrol agent as antagonist to a single pest or pathogen. This accounts for the inconsistency in the performance of biocontrol agents. The development of a bioformulation possessing a mixture of bioagents could be a viable option for the management of major pests and diseases in crop plants.
A bioformulation containing a mixture of Beauveria bassiana (B2) and Bacillus subtilis (EPC8) was tested against Fusarium wilt and fruit borer in tomato under glasshouse and field conditions. The bioformulation with B2 and EPC8 isolates effectively reduced the incidence of Fusarium wilt (Fusarium oxysporum f. sp. lycopersici) and fruit borer (Helicoverpa armigera) under glasshouse and field conditions compared with the individual application of B2 and EPC8 isolates and control treatments. In vitro studies showed a higher larval mortality of H. armigera when fed with B2 + EPC8-treated leaves. Further, plants treated with the B2 + EPC8 combination showed a greater accumulation of defence enzymes such as lipoxygenase, peroxidase and polyphenol oxidase against wilt pathogen and fruit borer pest than the other treatments. Moreover, a significant increase in growth parameters and yield was observed in tomato plants treated with B2 + EPC8 compared with the individual bioformulations and untreated control.
The combined application of Beauveria and Bacillus isolates B2 and EPC8 effectively reduced wilt disease and fruit borer attack in tomato plants. Results show the possibility of synchronous management of tomato fruit borer pest and wilt disease in a sustainable manner.
大多数病虫害生物防治方法都使用单一生物防治剂来对抗单一害虫或病原体。这导致了生物防治剂性能的不一致。开发一种含有多种生物制剂混合物的生物制剂配方可能是管理作物主要病虫害的可行选择。
在温室和田间条件下，对含有球孢白僵菌（B2）和枯草芽孢杆菌（EPC8）混合物的生物制剂配方进行了针对番茄枯萎病和棉铃虫的测试。与单独施用B2和EPC8分离株以及对照处理相比，含有B2和EPC8分离株的生物制剂配方在温室和田间条件下有效降低了枯萎病（尖孢镰刀菌番茄专化型）和棉铃虫（棉铃虫）的发病率。体外研究表明，用B2 + EPC8处理过的叶片喂养棉铃虫时，其幼虫死亡率更高。此外，与其他处理相比，用B2 + EPC8组合处理的植物对枯萎病病原体和棉铃虫害虫表现出更高的防御酶（如脂氧合酶、过氧化物酶和多酚氧化酶）积累。此外，与单独的生物制剂配方和未处理的对照相比，用B2 + EPC8处理的番茄植株的生长参数和产量显著增加。
球孢白僵菌和枯草芽孢杆菌分离株B2和EPC8的联合应用有效降低了番茄植株的枯萎病和棉铃虫侵害。结果表明以可持续方式同步管理番茄棉铃虫害虫和枯萎病的可能性。
