Gao Xiang, Kim Keun-Sik, Liu Dexi
Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA.
AAPS J. 2007 Mar 23;9(1):E92-104. doi: 10.1208/aapsj0901009.
Gene delivery using nonviral approaches has been extensively studied as a basic tool for intracellular gene transfer and gene therapy. In the past, the primary focus has been on application of physical, chemical, and biological principles to development of a safe and efficient method that delivers a transgene into target cells for appropriate expression. This review summarizes the current status of the most commonly used nonviral methods, with an emphasis on their mechanism of action for gene delivery, and their advantages and limitations for gene therapy applications. The technical aspects of each delivery system are also reviewed, with a focus on how to achieve optimal delivery efficiency. A brief discussion of future development and further improvement of the current systems is intended to stimulate new ideas and encourage rapid advancement in this new and promising field.
作为细胞内基因转移和基因治疗的一种基本工具，使用非病毒方法进行基因递送已得到广泛研究。过去，主要关注点在于应用物理、化学和生物学原理来开发一种安全有效的方法，将转基因递送至靶细胞以实现适当表达。本综述总结了最常用的非病毒方法的现状，重点阐述了它们的基因递送作用机制以及在基因治疗应用中的优缺点。还对每个递送系统的技术方面进行了综述，重点关注如何实现最佳递送效率。对当前系统未来发展和进一步改进的简要讨论旨在激发新想法，并鼓励这一新兴且有前景的领域快速发展。
