Kaneshima Takashi, Hohdatsu Tsutomu, Hagino Ryoko, Hosoya Sakiko, Nojiri Yui, Murata Michiko, Takano Tomomi, Tanabe Maki, Tsunemitsu Hiroshi, Koyama Hiroyuki
Department of Veterinary Infectious Disease, School of Veterinary Medicine and Animal Science, Kitasato University, Japan.
J Vet Med Sci. 2007 Mar;69(3):301-3. doi: 10.1292/jvms.69.301.
Canine respiratory coronavirus (CRCoV), which is more closely related to the bovine coronavirus (BCoV), has recently been detected in dogs. In this study, we examined whether BCoV was capable of infecting and exhibiting pathogenicity in dogs. Three 1-month-old pups were oronasally given field isolates of BCoV, and were kept together with 2 control animals. As a result, increases in BCoV-neutralizing antibody titers were confirmed in all pups in the challenged and control groups. Moreover, the virus gene was also detected in oral and rectal swabs by RT-PCR. These results indicate that BCoV infects dogs, and easily infects other dogs that are kept together. However, no clinical symptoms such as respiratory symptoms and diarrhea were observed.
犬呼吸道冠状病毒（CRCoV）与牛冠状病毒（BCoV）关系更为密切，最近在犬类中被检测到。在本研究中，我们检测了BCoV是否能够感染犬类并表现出致病性。给三只1月龄幼犬经口鼻腔接种BCoV的野外分离株，并与两只对照动物饲养在一起。结果，在攻击组和对照组的所有幼犬中均确认了BCoV中和抗体滴度升高。此外，通过RT-PCR在口腔和直肠拭子中也检测到了病毒基因。这些结果表明BCoV可感染犬类，并且容易感染饲养在一起的其他犬类。然而，未观察到诸如呼吸道症状和腹泻等临床症状。
