Stummer Moritz, Frisch Vicky, Glitz Frauke, Hinney Barbara, Spergser Joachim, Krücken Jürgen, Diekmann Irina, Dimmel Katharina, Riedel Christiane, Cavalleri Jessika-Maximiliane V, Rümenapf Till, Joachim Anja, Lyrakis Manolis, Auer Angelika
Institute of Virology, University of Veterinary Medicine, 1210 Vienna, Austria.
Clinical Unit of Equine Internal Medicine, University of Veterinary Medicine, 1210 Vienna, Austria.
Pathogens. 2023 Aug 15;12(8):1043. doi: 10.3390/pathogens12081043.
Acute abdominal pain (colic) is one of the major equine health threats worldwide and often necessitates intensive veterinary medical care and surgical intervention. Equine coronavirus (ECoV) infections can cause colic in horses but are rarely considered as a differential diagnosis. To determine the frequency of otherwise undetected ECoV infections in horses with acute colic, fresh fecal samples of 105 horses with acute colic and 36 healthy control horses were screened for viruses belonging to the species by RT-PCR as well as for gastrointestinal helminths and bacteria commonly associated with colic. Horses with colic excreted significantly fewer strongyle eggs than horses without colic. The prevalence of anaerobic, spore-forming, gram-positive bacteria ( and ) was significantly higher in the feces of horses with colic. Six horses with colic (5.7%) and one horse from the control group (2.8%) tested positive for Betacoronaviruses. Coronavirus-positive samples were sequenced to classify the virus by molecular phylogeny (N gene). Interestingly, in three out of six coronavirus-positive horses with colic, sequences closely related to bovine coronaviruses (BCoV) were found. The pathogenic potential of BCoV in horses remains unclear and warrants further investigation.
急性腹痛（绞痛）是全球马健康面临的主要威胁之一，通常需要 intensive veterinary medical care 和手术干预。马冠状病毒（ECoV）感染可导致马出现绞痛，但很少被视为鉴别诊断。为了确定急性绞痛马中未被检测到的 ECoV 感染频率，对 105 匹急性绞痛马和 36 匹健康对照马的新鲜粪便样本进行了 RT-PCR 筛查，以检测属于该物种的病毒，以及与绞痛常见相关的胃肠道蠕虫和细菌。患有绞痛的马排出的圆线虫卵明显少于无绞痛的马。厌氧、产芽孢、革兰氏阳性细菌（和）在患有绞痛的马粪便中的患病率显著更高。6 匹患有绞痛的马（5.7%）和 1 匹对照组马（2.8%）检测出β冠状病毒呈阳性。对冠状病毒阳性样本进行测序，通过分子系统发育（N 基因）对病毒进行分类。有趣的是，在 6 匹冠状病毒阳性的绞痛马中，有 3 匹发现了与牛冠状病毒（BCoV）密切相关的序列。BCoV 在马中的致病潜力尚不清楚，值得进一步研究。
