Skogerson L, Wakatama E
Proc Natl Acad Sci U S A. 1976 Jan;73(1):73-6. doi: 10.1073/pnas.73.1.73.
Three proteins required for poly(U)-directed polyphenylalanine synthesis have been separated from yeast. Two of the factors correspond to the elongation factors 1 and 2 described for other eukaryotic systems, according to the criteria of phenylalanyl-tRNA binding and diphtheria toxin-catalyzed ADP-ribosylation. The third protein, while absolutely required for polyphenylalanine synthesis, was a more active ribosome-dependent GTPase than elongation factor 2.
已从酵母中分离出聚(U)指导的聚苯丙氨酸合成所需的三种蛋白质。根据苯丙氨酰 - tRNA结合和白喉毒素催化的ADP核糖基化标准,其中两种因子对应于其他真核系统中描述的延伸因子1和2。第三种蛋白质虽然是聚苯丙氨酸合成绝对必需的,但它是一种比延伸因子2更具活性的核糖体依赖性GTP酶。